Method Submissions-5th Set of Nutrients_6-30-14 - page 58

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AppendixC
Definition of enzyme activity
Sucrase:system name is β-D-furan fructo-fructose hydrolase, EC 3.2.1.26, one enzyme activityunit
is defined as the enzyme amount needed for hydrolysis production of 1μmol glucose everyminute
under optimum reaction conditions.
β-amylase: system name is α-1,4-glucan hydrolasemaltose, EC 3.2.1.2, one enzyme activityunit
is defined as the enzyme amount needed for hydrolyzing starch and producing1μmolmaltose every
minute under optimum reaction conditions.
Pullulanase: system name is amylopectin-6-glucanohydrolase, EC 3.2.1.41, one enzyme activity
unit is defined as the enzyme amount needed for hydrolyzing amylopectin and producing1μmol
reducing sugar everyminute under optimum reaction conditions.
Maltase:system name is 4-O-α-D-glucopyranosyl group- (1→4)-α-D-glucopyranose, EC 3.2.1.20,
one enzyme activityunit is defined as the enzyme amount needed for hydrolyzingmaltose and
producing1μmol glucose everyminute under optimum reaction conditions.
Inulase: system name is β-2,1-D-fructan hydrolase, exo-inulinaseEC 3.2.1.80, endo-inulinaseEC
3.2.1.7, one enzyme activityunit is defined as the enzyme amount needed for producing1μmol
reducing sugar (measured by fructose) everyminute under optimum reaction conditions.
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