Therefore,
5. 412
1
1
1
p k
mA
CVA
C
s
s
Where:
C=Concentrationof fructan in the sample inmg/100g
A
1
=Areaof sample fructosepeak
A
s
=Areaof standard fructosepeak
V
1
=Volumeof sample solution (inmL), normally100mL
m
1
=weight of sampleused toprepare the sample solution (inmg), normally1000mg
C
s
=Concentrationof fructose standard (inμg/mL), normallyaround7μg/mL
k= correction factor forwater uptake
p= correction factor for lossof terminalmonosaccharide residue.
0.2
=
volumeof “sample filtrate” taken for hydrolysisof sucroseand starch (inmL)
1.1
=
total volumeof “solutionA” (inmL)
0.4
=
aliquot of “solutionA” taken for SPE cleanup (inmL)
0.3
=
volume inwhichdried fructans are resuspended for digestion (inmL)
100
=
conversion factor toexpress final result inmg/100g
FOS-03
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
