Figure 2: Configuration of 10-Port SwitchingValve
D.
Reagents
(a)
Dymethylsulfoxide, purissp.a.
(b)
Anthranilic acidamide, purum
(c)
Ammoniumacetate, p.a.
(d)
Sodium cyanoborohydride, purum
(e)
Formic acid,GR for analysis
(f)
AceticAcidanhydrous, GR for analysis
(g)
Acetonitrile,Gradient grade for LC
(h)
Ammoniumhydroxide solution, GR for analysis
(i)
18MΩwater isused throughout (referred toaswater)
(j)
Laminaritriose
(k)
Maltotriosemin. 95% (e.g. SigmaM8378; >95%)
(l)
Amyloglucosidase fromaspergilusniger; Roche: 11202367001
(m)Maltotriose standard stock solution (3.0µmol/mL):Weigh 75mg
5mg ofmaltotriose and
record the mass to 0.1mg. Dissolve with 40mL of water in a 50-mL volumetric flask and
complete to themarkwithwater.
(n)
Maltotriose standardworking solution (0.30µmol/mL): Pipette10.0mL ofmaltotriose stock
solution (m) ina100-mL volumetric flaskand complete to themarkwithwater.
(o)
Laminaritriose internal standard stock solution (1.2µmol/mL): Weigh 15mg
3mg of
Laminaritriose and record the mass to 0.1mg. Dissolve with 15 mL of water in a 25-mL
volumetric flaskand complete to themarkwithwater.
(p)
Laminaritriose internal standard working solution (0.3µmol/mL): Pipette 5.0mL of
Laminaritriose stock solution (o) in a 20-mL volumetric flask and complete to themarkwith
water.
GOS-01
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
