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Eluents
Fractionation
HPLC eluent A 1000mMNaOH
Transfer 1900mLMilli-Q into a plastic eluent bottle, while flushingwith
nitrogen. Add immediately 104mL 50%NaOH solution, while flushingwith
nitrogen. Close the bottlewith the eluent lid and homogenize.
HPLC eluent B 1000mMNaAC
Use one ampul of 82.04 gram NaAc, dissolve and adjust to 1 liter usingMili-Q.
Transfer into in a plastic eluent bottle.
HPLC Eluent DMilli-Q
Quantification
HPLC eluent A 300mMNaOH
Add 1900mL Milli-Q in a plastic eluent bottle, while flushingwith nitrogen.
Add immediately 30.3mL 50%NaOH solution, while flushingwith nitrogen.
Close the bottlewith the eluent lid and homogenize
HPLC eluent B 1000mM NaAC
Use one ampul of 82.04 gram NaAc, dissolve and adjust to 1 liter usingMili-Q.
Transfer into in a plastic eluent bottle.
HPLC Eluent DMilli-Q
Reagentia
0.2MPotassiumphosphatebuffer pH6.0±0.1
Weigh 13.60 gram KH
2
PO
4
and dissolve in 500mL Milli-Q
Weigh 6.96 gram K
2
HPO
4
and dissolve in 200mLMilli-Q
Prepare a buffer solution of pH 6.0 ± 0.1 by adding K
2
HPO
4
solution to the
KH
2
PO
4
solution.
Dilute 5x times before use (=40mM). Store in the refridgerator.
Carrez reagent 1
Weigh 17.2 gram K
4
Fe(CN)
6
.3H
2
O
and dissolve in 100mLMilli-Q. Store the
solution in the refrigerator.
Carrez reagent 2
Weigh 53.5 gram ZnSO
4
.7H
2
O and dissolve in 100mLMilli-Q. Store the solution
in the refrigerator.
Beta-galactosidase solution (400U/mL) (daily fresh)
Weigh 40mg enzyme and dissolve in 1mL 40mM potassium phosphate buffer
80%Acetonitrile/ 0.1% TFA
Transfer 80mL 100% Acetonitrile into a beaker
Add 20mL Milli-Q
Add 0.1mL TFA
Mix and store in a closed bottle
25%Acetonitrile
Transfer 25mL 100% Acetonitrile to a beaker
Add 75mL Milli-Q
Mix and store in a closed bottle
GOS-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
