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Material
2L, 50mL glassmeasuring flasks
Various beakers
2mL safelock tubes (e.g. Eppendorf, VWR p/n. 211-2120)
Pasteur pipettes (e.g. VWR p/n. 612-1684)
Microfilters: 0,2 µm (e.g. GHP Acrodisc 13, VWR p/n. 516-6916)
2mL syringe (e.g. VWR p/n. 613-3911)
HPLC vials 2mL with split septum suitable for the ICS used
HPLC vials 300 uL with split septum (e.g. TPX Short treadMicro-Vials, Grace
Davison p/n. 5133602)
15mL tubes (e.g. Falcon, VWR p/n. 734-0452)
Carbograph SPE extract clean 150mg column (Grace Davison p/n. 210142)
Apparatus
Centrifuge for 2mL safelock tubes (up to 13.000 rpm) (e.g. Eppendorf 5415D)
Analytical Balance accurate to 0.1mg (e.g. Mettler Toledo XS205)
pHmeter (e.g. Mettler Toledo SevenMulti)
Vortexmixer (e.g. Minishaker, IKAMS2)
Dionex-HPLC equipment ICS-5000 or equal, with:
Pump, 1.0mL/min (ICS5000+DP)
Thermostatic autosampler (ICS5000+ AS-AP)
Carbopac PA1 column (4*250mm) (Dionex p/n. 35391)
Carbopac PA1 guard column (4*50mm) (Dionex p/n. 43096)
Carbopac PA100 column (4*250mm) (Dionex p/n. 43055)
Carbopac PA100 guard column (4*50mm) (Dionex p/n. 043054)
ED detector PAD, with standard carbohydrate waveform for Ag/AgCl ref
electrode or Pd ref electrode
Au electrode for carbohydrates
Speedvac (e.g. Eppendorf concentrator 5301)
Waterbath 60 ˚C and 100°C (e.g. grant GLS 200
Vac-elute sps-24with pump
Chemicals
Highly purifiedwater, (Milli-Q)
Sodiumacetate anhydrous(C
2
H
3
NaO
2
) (Thermo Scientific p/n. 059326)
Sodiumhydroxide opl. 50% (VWR p/n. BAKR3727-1000)
D(+)-Galactose (Sigma p/n. G-0750)
D(+)-Glucose (Sigma p/n. G-8270)
Lactosemonohydrate (Sigma p/n. 61339)
Ribose (Sigma p/n. R7500) or other suitable internal standard
Helium
Carrez reagent
Carrez 1: K
4
Fe(CN)
6
.3H
2
O (VWR p/n. 1.04984.0500)
Carrez 2: ZnSO
4
.7H
2
O (VWR p/n. 1.08883.1000)
1M phosphate buffer pH 6.0 ± 0.1
KH
2
PO
4
(VWR p/n. 1.04871.1000)
K
2
HPO
4
(VWR p/n. 1.05101.1000)
Beta Galactosidase from Aspergillus Oryzae (Sigma p/n. G1560 15 KU)
Acetonitrile (VWR p/n. 1000302500)
Trifuor Acetic Acid, (TFA) (Thermo Scientific p/n. 28904)
GOS-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
