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Method: GOS fractionation
Colomn PA100
Eluent A= 1MNaOH
Eluent B= 1MNaAC
Eluent D=Milli-Q
Flow 1mL/min
Column temperature 20°C
Tray temperature 10°C
Pressure upperlimit = 4000 Psi
Sample injection 25 µl
Time (min) Eluent A
(%)
Eluent B
(%)
Eluent C
(%)
Eluent D
(%)
Curve
0.00
10
4
0
86
5
1.10
10
4
0
86
5
4.00
50
40
0
10
5
8.00
50
40
0
10
5
8.10
10
4
0
86
5
18.0
10
4
0
86
5
Method: GOSquantification
Column PA1
Eluent A= 300mMNaOH
Eluent B= 1MNaAC
Eluent D=Milli-Q
Flow 1mL/min
Column temperature 20°C
Tray temperature 10°C
Pressure upperlimit = 3000 Psi
Sample injection 25 µl
Time (min) Eluent A
(%)
Eluent B
(%)
Eluent C
(%)
Eluent D
(%)
Curve
0
5
0
0
95
5
20
5
0
0
95
5
33
32
0
0
68
5
35
32
0
0
68
5
39
32
50
0
18
5
43
32
50
0
18
5
45
32
0
0
68
5
48
5
0
0
95
5
63
5
0
0
95
5
GOS-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
