OSmolar conc. =Molar concentrationof the individual oligosaccharide in the sample test calculated
with theequation1as: C_OSsple.
MW=Molecularweight of thedifferent analytes ing/mol (assignedbyMS)
0.0001= Factor to convertµg/g tog/100g
Note: Do not include lactose, maltose (resulting from starch orMD hydrolysis) or laminaritriose IS in
the sumof total oligosaccharides.
K.
Performance
Sample
Average
(g/100g)
(a)
NoofDays x
replicates
S
r
RSD
r
(%)
S
iR
RSD
iR
(%)
Recovery
(%)
Formula
(1
st
stage)
2.70
8 x2
0.021
0.8
0.031
1.1
n/a
Formula
(2
nd
stage)
2.39
8 x2
0.010
0.4
0.048
2.0
n/a
GOS
Ingredient
43.1
8 x2
0.260
0.6
0.47
1.1
96.6
(b)
Spiked
formula
2.63
1 x6
0.010
0.4
n/a
n/a
102
Spiked
formula
5.04
1 x6
0.014
0.3
n/a
n/a
97.5
Spiked
formula
9.95
1 x6
0.186
1.9
n/a
n/a
96.5
SpikedHA
formula
2.42
1 x6
0.023
0.9
n/a
n/a
93.9
SpikedHA
formula
4.94
1 x6
0.012
0.3
n/a
n/a
95.5
SpikedHA
formula
9.80
1 x6
0.107
1.1
n/a
n/a
94.9
(a)Quantity ing/100gof product as-received (i.e. inpowdered formulaor inGOS syrup). (b) compared to
current AOAC2001.02method
GOS-01
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
