Pagina 10 van 11
annex 9.3
Descriptionmethod
The fraction is dried in the speedvac. Residue is takenup inbuffer and divided over two test tubes.
After incubation the galactose and lactose content is determined byHPAEC-PED analysis.
reproducibility
date
n
recovery (%)
stdev
60404
GOS
2
93
2
60630
GOS
3
82
6
60829
GOS
2
96
2
date
n
g/100gproduct
stdev stdev(%)
60713
std1
5
5,3
0,2
4
60713
std2
5
4,7
0,7
15
60713
IMFGOS
5
5,2
0,7
14
60713
std2
5
4,8
0,2
4
60706
immunocomplete
3
4,9
0,1
2
repeatability
60630
60706
60713
60829
average stdev(%)
IMFGOS (%)
96
107
98
102
101
5
immunocomplete (g/100g) 4,4
4,9
4,9
5,1
5
6
Dosis/repons
min.
max.
GOS
2,5
10
Stability of reagens:
reagent
max. utility
standards
6months stored inportions in freezer
NaOH eluent
1month if kept under helium atmosphere
NaAc eluent
1month if kept under helium atmosphere
samples
suitability:
date
n average (g/100gprod)) stdev
Immunocomplete
60829
2
5,1
0,3
std1
60829
2
5,3
0,1
std2
60829
2
5,2
0,0
Spiking:
spike
IMF
GOS (mg)
recovery(%)
60630 41
100
41
91
60706 94
107
93
88
60829 68
99
68
105
Validation report
range (g/100gproduct)
2,5-10
The determinationofGalacto oligosaccharide in infantmilk formula
Sample is disolved inwater and after treatment withCarrez a SPE step is performed using aCarbograph column
One is incubatedwith active and the otherwith in-active enzyme.
GOS-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
