1000
b s
a s
s
VV
Vm
C
Where:
Cs =Concentrationof fructose standard solution (inμg/mL)
m
s
=Weight of fructoseused tomake the fructose stock solution (inmg), normallyabout
100mg
V
a
=Volumeof fructose stock solutionused toprepare the fructose standard solution (inmL),
normallyabout 0.7mL
V
b
=Final volumeof the fructose stock solution (inmL), normally100mL
V
s
= final volumeof the fructose standard solution (inmL), normally100mL
1000= factor to convertmg/mL intoμg/mL
The correction factors kandp canbe calculated from the ingredientDPas follows:
DP
DP
k
180
))1 (
162 (
180
1
DP
DP
p
Where:
DP=averagedegreeof polymerisationof the fructan (i.e. theaveragenumber of
monosaccharide residues per chain).
k= correction factor for theuptakeofwater duringhydrolysis.
p= correction factor to correct for lossof terminal fructose inF
m
type fructansor terminal
glucose inGF
n
type fructans.
The concentrationof fructan in theproduct canbe calculatedas follows:
100
4.02.0
3.01.1
1
1
1
p k
mA
CVA
C
s
s
FOS-03
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
