and re-equilibrated for 10minwith5.1mM sodiumhydroxidebefore thenext injection (see
tablebelow for details).
Post columnaddition
- 300mM sodiumhydroxide is introducedat a flow rateof 0.6mL/min
viaaT-piecebetween the columnand thePADdetector.
Injection
–A single20μL injection ismadeof each sample test solution. At the start of each
series twoblank (water) injections of 20μL aremade to stabilise the system, followedbya
20μL injectionof standard solution. A20μL injectionof the standard solution ismadeafter
every6 sample injections andat theendof the series.
Retenetion times
–Retenetion timesare controlledby comparing that of the standardwith
that of the sample. Typically fructoseelutes at around36minutes.
Method for LCdeterminationof fructose
Time (min)
%A (NaOH, 300mM)
%B (water)
Comment
0
1.7
98.3
Start
45
1.7
98.3
Finish isocraticelution
45.1
100
0
Start columnwash
55.0
100
0
End columnwash
55.1
1.7
98.3
Start re-equilibation
65.0
1.7
98.3
End re-equilibration
G.
Calculations
The concentrationof the fructose standard is calculatedas follows:
FOS-03
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
