AOAC SPIFAN Review Team (February 2017)

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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals - APPENDICES
Jaudzems, Lahrichi, and Fuerer – January 2017 page 10/22
High concentration standard (50 pmol/μL, 1 μL injected). Full (top) and zoomed (bottom) views
1.667
AMQ - 2.795
NH3 - 3.284His - 3.653
mono-LYS - 4.555
Tau - 4.816 Ser - 6.128Arg - 7.060Gly - 7.523 Asp - 9.237 Glu - 12.026
12.826
Thr - 13.528Ala - 14.579
14.929
Pro - 15.728
16.00216.27616.637
Deriv Peak - 16.956
17.068
17.143
Lys - 17.262
Tyr - 17.630Met - 18.018Val - 18.291
NVa - 18.59518.808 19.558
Ile - 20.074Leu - 20.295Phe - 20.584
20.91521.36021.854
AU
0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40
1.60
Minutes
1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00
1.667
AMQ - 2.795
NH3 - 3.284
His - 3.653
mono-LYS - 4.555
Tau - 4.816
Ser - 6.128 Arg - 7.060Gly - 7.523 Asp - 9.237
Glu - 12.026
12.826
Thr - 13.528
Ala - 14.579
14.929
Pro - 15.728
16.00216.27616.637
Deriv Peak - 16.956
17.068
17.143
Lys - 17.262
Tyr - 17.630
Met - 18.018Val - 18.291
NVa - 18.595
18.808 19.558
Ile - 20.074Leu - 20.295Phe - 20.584
20.915 21.360
AU
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
0.22
0.24
0.26
0.28
0.30
0.32
0.34
0.36
0.38
Minutes
1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50
AMINO-01 (FEBRUARY 2017) - APPENDICES
FOR ERP USE ONLY
DO NOT DISTRIBUTE