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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals - APPENDICES

Jaudzems, Lahrichi, and Fuerer

January 2017

page 15/22

SPIFAN II SLV kit product #5: Infant Formula RTF, Milk Based (Placebo)

SPIFAN II SLV kit product #6: NIST SRM 1849A

2.212

AMQ - 2.977

NH3 - 3.573

His - 4.104

6.647

Ser - 6.925

Arg - 8.209

Gly - 8.481

8.960

Asp - 10.417

11.333

Glu - 12.768

13.396

Thr - 13.949

Ala - 14.878

Pro - 15.927

16.950

Deriv Peak - 17.078

Lys - 17.376

17.514

Tyr - 17.788

xCys - 18.061

Met - 18.163

Val - 18.412

NVa - 18.712

18.980

Ile - 20.172

Leu - 20.389

Phe - 20.701

20.994

21.876

AU

0.000

0.005

0.010

0.015

0.020

0.025

0.030

0.035

0.040

0.045

0.050

0.055

0.060

0.065

0.070

0.075

0.080

0.085

0.090

Minutes

1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

2.208

AMQ - 2.962

NH3 - 3.544

His - 4.055

Tau - 5.332

6.586

Ser - 6.820

Arg - 8.057

Gly - 8.355

8.844

Asp - 10.255

11.262

Glu - 12.676

13.328

Thr - 13.899

Ala - 14.843

Pro - 15.909

Deriv Peak - 17.070

Lys - 17.369

17.481

Tyr - 17.771

xCys - 18.045 Met - 18.149

Val - 18.402

NVa - 18.702

18.980

Ile - 20.163

Leu - 20.380

Phe - 20.688

20.992

21.874

AU

0.000

0.005

0.010

0.015

0.020

0.025

0.030

0.035

0.040

0.045

0.050

0.055

0.060

0.065

0.070

0.075

0.080

0.085

0.090

0.095

0.100

0.105

0.110

0.115

0.120

Minutes

1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

AMINO-01 (FEBRUARY 2017) - APPENDICES

FOR ERP USE ONLY

DO NOT DISTRIBUTE