AOAC SPIFAN Review Team (February 2017)

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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals - APPENDICES
Jaudzems, Lahrichi, and Fuerer – January 2017 page 9/22
Appendix I: example chromatograms for selected concentrations of the calibration curve and SPIFAN II SLV kit products.
Low concentration standard (0.25 pmol/μL, 1 μL injected). Full (top) and zoomed (bottom) views
1.670
AMQ - 2.779
NH3 - 3.291
His - 3.661 Tau - 4.825 Ser - 6.143 Arg - 7.095Gly - 7.543 Asp - 9.252 Glu - 12.037 Thr - 13.537 Ala - 14.584 Pro - 15.73115.803
Deriv Peak - 16.957
17.070Lys - 17.267Tyr - 17.632Met - 18.020Val - 18.29318.418
NVa - 18.597
Ile - 20.078Leu - 20.299Phe - 20.58820.91821.35921.658
21.855
AU
0.000
0.010
0.020
0.030
0.040
0.050
0.060
0.070
0.080
0.090
0.100
0.110
0.120
0.130
0.140
0.150
Minutes
1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00
1.670
AMQ - 2.779
NH3 - 3.291
His - 3.662 Tau - 4.825 Ser - 6.143 Arg - 7.095Gly - 7.543 Asp - 9.252
Glu - 12.037
Thr - 13.537
Ala - 14.584
Pro - 15.731
15.803
Deriv Peak - 16.957
17.070
Lys - 17.267
Tyr - 17.632Met - 18.020
Val - 18.293
18.418
NVa - 18.597
Ile - 20.078Leu - 20.299Phe - 20.588
20.918
AU
0.0000
0.0005
0.0010
0.0015
0.0020
0.0025
0.0030
0.0035
0.0040
0.0045
0.0050
0.0055
0.0060
0.0065
0.0070
Minutes
1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50
AMINO-01 (FEBRUARY 2017) - APPENDICES
FOR ERP USE ONLY
DO NOT DISTRIBUTE