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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals - APPENDICES
Jaudzems, Lahrichi, and Fuerer
–
January 2017
page 4/22
Appendix D
: repeatability data obtained with the SPIFAN II SLV kit products, color-coded with respect to performance requirement values.
SPIFAN II SLV kit product
Analyte
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
His
0.7%
0.4%
1.2%
3.7%
1.9%
0.5%
0.8%
0.9%
2.0%
1.1%
1.4%
1.2%
3.0%
1.2%
2.0%
1.4%
0.0%
1.6%
0.8%
Tau
N/A
N/A
N/A
N/A
N/A
1.2%
1.4%
1.8%
2.3%
1.3%
N/A
1.0%
1.9%
1.7%
2.8%
1.8%
1.6%
2.2%
2.5%
Ser
0.7%
0.6%
0.2%
0.7%
0.6%
1.0%
1.0%
0.7%
1.0%
0.8%
0.4%
0.8%
2.9%
1.9%
2.0%
0.5%
0.7%
1.5%
1.5%
Arg
0.5%
0.4%
0.8%
1.9%
1.5%
1.3%
1.4%
1.1%
1.5%
1.3%
1.9%
1.6%
1.7%
1.7%
1.2%
1.3%
1.5%
1.6%
2.0%
Gly
0.6%
1.1%
1.0%
1.7%
1.9%
0.4%
1.2%
1.2%
1.4%
0.4%
1.0%
1.0%
1.2%
0.7%
0.5%
0.5%
0.0%
1.0%
1.5%
Asp
0.9%
1.5%
1.4%
2.3%
0.7%
0.5%
0.4%
0.7%
0.8%
1.2%
0.9%
0.4%
1.7%
0.6%
1.8%
0.5%
0.7%
0.6%
1.1%
Glu
0.6%
0.6%
0.6%
2.0%
1.0%
0.5%
0.3%
0.8%
0.6%
0.7%
0.5%
0.6%
1.4%
0.4%
0.5%
0.7%
0.5%
0.6%
1.6%
Thr
0.3%
0.6%
0.4%
0.6%
0.6%
0.7%
0.8%
0.7%
0.7%
1.1%
0.4%
0.5%
1.6%
1.2%
1.3%
0.4%
0.6%
1.0%
1.3%
Ala
0.5%
0.9%
1.2%
1.7%
0.8%
0.7%
0.3%
0.8%
0.7%
0.7%
0.4%
0.5%
1.6%
0.4%
0.5%
0.3%
0.8%
0.7%
1.3%
Pro
0.4%
0.3%
0.5%
1.4%
0.9%
0.8%
0.5%
1.3%
0.6%
0.7%
0.8%
0.4%
1.2%
0.6%
0.7%
0.4%
0.0%
0.8%
1.0%
Lys
1.0%
0.6%
0.5%
3.0%
0.9%
1.0%
0.7%
1.4%
1.2%
0.5%
1.0%
1.3%
1.9%
0.9%
0.9%
0.7%
0.9%
0.9%
1.5%
Tyr
0.5%
1.6%
1.6%
1.8%
0.9%
0.8%
0.6%
1.5%
1.5%
1.7%
1.3%
0.6%
2.4%
0.3%
1.0%
1.3%
1.9%
1.2%
1.0%
xCys
1.0%
0.5%
0.9%
3.1%
0.0%
1.6%
1.2%
1.4%
1.1%
2.1%
1.7%
1.3%
3.9%
0.7%
0.6%
2.9%
0.0%
0.0%
2.1%
Met
0.3%
0.7%
0.8%
1.6%
1.7%
1.5%
1.4%
1.0%
1.1%
1.0%
1.1%
1.3%
0.7%
0.8%
0.6%
1.0%
0.0%
1.5%
2.1%
Val
2.0%
0.3%
1.1%
1.5%
2.6%
0.8%
1.2%
1.3%
2.0%
1.7%
0.7%
0.8%
0.9%
1.8%
1.6%
3.4%
1.8%
1.9%
1.1%
Ile
1.7%
0.3%
1.3%
1.9%
3.4%
0.9%
0.7%
1.1%
2.2%
2.1%
0.7%
0.8%
1.2%
2.2%
2.3%
3.2%
2.5%
2.3%
1.7%
Leu
0.5%
0.3%
0.5%
1.4%
1.0%
0.9%
0.5%
0.7%
1.2%
0.5%
0.5%
0.5%
1.0%
0.6%
0.7%
0.9%
0.4%
0.8%
1.0%
Phe
0.7%
0.7%
1.2%
2.0%
1.8%
0.6%
1.1%
1.0%
1.9%
1.1%
0.8%
1.1%
1.2%
0.5%
1.1%
0.7%
1.8%
0.8%
0.9%
Repeatability data were obtained with robust statistics.
For each analyte/matrix pair, the repeatability was compared with the corresponding performance requirement. 93% of the values are within the
requirements (green) and 5% outside (red). 2% could not be attributed (blank values for taurine).
AMINO-01 (FEBRUARY 2017) - APPENDICES
FOR ERP USE ONLY
DO NOT DISTRIBUTE