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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals - APPENDICES
Jaudzems, Lahrichi, and Fuerer
–
January 2017
page 1/22
Appendix A
: average values of the SPIFAN II SLV kit products in mg/100 g reconstituted product.
SPIFAN II SLV kit product
Analyte
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
His
41.2
45.3
126.5 195.8
27.0
34.6
22.2
31.9
35.5
27.2
44.9
37.9
44.2
28.0
25.6
33.6
28.0
129.0 189.0
Tau
N/A
N/A
N/A
N/A
N/A
4.1
4.7
5.2
3.5
4.7
N/A
8.1
4.0
3.8
4.7
6.4
4.7
17.5
20.1
Ser
98.2
62.3 307.8 448.8
82.3
78.4
71.6
73.3
87.5
65.9
71.0
88.9
57.9
66.8
66.3
75.2
79.3 312.0 461.8
Arg
63.6 105.4 709.3 244.0
35.8
43.3
29.8
94.4
46.3
35.2
87.2
59.3 103.8
36.8
34.1
100.7
36.0
707.5 237.0
Gly
36.3
47.3
106.0 141.8
28.0
27.0
24.7
56.5
29.9
26.6
153.3
35.4
48.8
25.2
22.5
58.3
28.0
106.3 141.0
Asp
158.1 198.1 503.0 581.3 155.8 126.2 159.4 173.4 147.9 121.4 143.5 147.1 195.9 127.3 113.9 173.5 145.8 491.8 583.3
Glu
363.5 244.0 1237.8 1789.0 302.5 305.4 241.5 279.1 334.3 209.8 243.4 334.9 239.9 248.5 244.4 281.4 287.8 1205.8 1785.5
Thr
79.6
79.7 252.5 326.5
87.0
70.3
93.1
52.8
79.1
61.1
69.5
75.4
78.9
65.7
66.3
54.0
85.8 252.8 328.5
Ala
64.3
64.6 218.8 240.8
65.3
52.2
69.5
61.9
61.3
40.6
89.5
60.4
62.5
53.1
49.7
61.5
62.3 214.8 239.5
Pro
146.4
61.4 519.0 840.5 116.3 129.8
77.6
70.4 134.7
79.0 129.2 136.2
60.8
93.1
97.0
72.1 113.5 510.8 835.8
Lys
143.4 119.8 491.0 594.8 121.0 120.8 131.6
89.3 141.4 100.4
98.0 132.0 111.6 112.6 100.2
87.5 115.5 484.8 597.8
Tyr
75.6
93.2 267.5 398.0
55.8
64.3
37.1
49.7
68.7
57.3
69.1
72.1
95.6
52.7
49.9
52.4
56.3 271.3 403.0
xCys
17.7
22.4
59.3
26.9
23.3
14.6
33.5
16.4
20.4
24.9
16.8
17.6
23.9
23.5
18.4
16.1
22.8
56.5
25.3
Met
38.1
41.3
138.8 196.3
31.5
51.0
25.6
34.1
36.6
22.3
37.3
36.0
43.0
29.6
28.6
41.4
32.0
141.5 199.5
Val
96.9 141.4 326.0 489.4
81.8
90.8
69.0
56.5
88.9
64.9
98.4
90.7 142.5
74.1
68.8
62.3
88.3 324.0 477.8
Ile
84.6 122.8 274.3 380.9
76.8
79.7
74.4
56.0
78.5
59.2
86.8
80.5 124.6
68.8
63.4
62.6
84.8 270.0 372.3
Leu
164.6 203.3 584.3 724.8 150.3 139.9 138.3 109.0 157.5 111.6 140.6 155.5 200.7 133.3 120.6 111.3 148.8 574.5 723.0
Phe
79.2 103.1 258.8 397.3
59.3
65.5
42.0
69.0
68.9
49.4
78.0
75.5 104.9
54.7
51.1
74.1
58.8 260.5 388.3
Values are in mg/100 g reconstituted product.
Following the SMPR, analyte/matrix pairs were categorized into three groups:
- 0.4-5.0 mg/100 g reconstituted product (red)
- 5.0-50 mg/100 g reconstituted product (orange)
- 50-2500 mg/100 g reconstituted product (green)
Those groups determine the performance requirement for each analyte/matrix pair according to the SMPR.
AMINO-01 (FEBRUARY 2017) - APPENDICES
FOR ERP USE ONLY
DO NOT DISTRIBUTE