AOAC SPIFAN Review Team (February 2017)

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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals - APPENDICES
Jaudzems, Lahrichi, and Fuerer – January 2017 page 2/22
Appendix B: average values of the SPIFAN II SLV kit products in mg/100 g product.
SPIFAN II SLV kit product
Analyte 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
His 371 407 127 196 27 311 200 287 319 245 404 342 398 252 231 303 28 129 189
Tau N/A N/A N/A N/A N/A 37 42 47 32 42 N/A 73 36 34 43 58 5 17 20
Ser 884 561 308 449 82 706 644 660 788 593 639 801 521 601 597 677 79 312 462
Arg 572 949 709 244 36 390 269 850 417 317 785 534 934 332 307 906 36 708 237
Gly 327 426 106 142 28 243 222 509 270 240 1380 319 440 227 203 524 28 106 141
Asp 1423 1783 503 581 156 1136 1435 1561 1331 1092 1292 1324 1764 1145 1025 1562 146 492 583
Glu 3272 2196 1238 1789 303 2749 2174 2512 3008 1888 2191 3014 2159 2236 2200 2533 288 1206 1786
Thr 717 717 253 327 87 633 838 476 712 550 626 679 710 591 596 486 86 253 329
Ala 579 581 219 241 65 470 626 558 552 365 806 544 563 478 447 554 62 215 240
Pro 1318 553 519 841 116 1168 698 633 1213 711 1163 1226 548 838 873 649 114 511 836
Lys 1290 1078 491 595 121 1088 1184 803 1273 903 882 1188 1004 1014 902 788 116 485 598
Tyr 681 839 268 398 56 579 334 447 619 516 622 649 860 474 450 471 56 271 403
xCys 159 202 59 27 23 132 302 148 184 224 151 159 215 211 166 145 23 57 25
Met 343 372 139 196 32 459 230 307 330 201 335 324 387 267 257 373 32 142 200
Val 872 1272 326 489 82 817 621 508 800 584 886 816 1283 667 619 561 88 324 478
Ile 762 1105 274 381 77 718 670 504 707 533 781 725 1121 620 571 563 85 270 372
Leu 1482 1830 584 725 150 1259 1245 981 1418 1004 1266 1399 1807 1200 1086 1001 149 575 723
Phe 713 928 259 397 59 589 378 621 620 445 702 680 944 492 460 667 59 261 388
Values are in mg/100 g product (powder or ready-to-feed liquid).
The color code follows that on Appendix A and is based on the concentrations in the reconstituted final product according to the SMPR.
AMINO-01 (FEBRUARY 2017) - APPENDICES
FOR ERP USE ONLY
DO NOT DISTRIBUTE