47 / 164
Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals
Jaudzems, Lahrichi, and Fuerer
–
January 2017
page 19/20
Two products show strong matrix effects with bias towards either low (#4) or high (#12)
recoveries.
Finally, recoveries for Product 6 (NIST 1849A) were compared with the reference values
from the certificate of analysis (CoA).
4.10
Chromatograms
Example chromatograms for selected concentrations of the calibration curve and SPIFAN II
SLV kit products are presented in Appendix I.
Analyte
6
(spike)
6
(CoA)
His
92.6% 99.7%
Tau
97.0% 100.2%
Ser
104.0% 98.0%
Arg
94.8% 97.4%
Gly
97.9% 100.7%
Asp
105.2% 106.2%
Glu
103.4%
106.1%
Thr
99.6%
124.1%
Ala
99.5% 103.2%
Pro
100.2% 97.7%
Lys
100.6%
107.7%
Tyr
96.1%
113.4%
xCys
99.4% 102.4%
Met
95.4% N/A
Val
91.6% 107.5%
Ile
89.8% 108.7%
Leu
96.8% 99.9%
Phe
96.8% 101.6%
AMINO-01 (FEBRUARY 2017)
SLV REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE