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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals - APPENDICES
Jaudzems, Lahrichi, and Fuerer
–
January 2017
page 6/22
Appendix F
: intermediate reproducibility data obtained with the SPIFAN II SLV kit products, color-coded with respect to reproducibility
requirement values.
SPIFAN II SLV kit product
Analyte
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
His
4.9%
7.8%
4.6% 11.0% 6.8%
4.7%
7.5% 10.0% 9.2%
5.6%
7.6%
6.4%
3.7%
6.3% 12.0% 7.5%
4.3%
7.5%
9.3%
Tau
N/A
N/A
N/A
N/A
N/A
2.3%
4.2%
4.2% 10.2%
2.2%
N/A
6.3%
7.1%
3.7%
7.7%
6.6%
6.2%
3.9%
4.4%
Ser
7.4% 10.6% 6.9%
5.7%
5.8%
9.0%
4.2%
6.7%
2.3%
2.4%
3.5%
7.4%
6.3%
3.0%
5.0%
7.9%
5.3%
8.8%
9.7%
Arg
3.7%
2.1%
2.0%
7.9%
7.6%
2.7%
6.4%
3.0%
8.1%
6.4%
5.1%
8.2%
4.3%
6.4%
8.0%
8.1%
2.0%
6.6%
7.2%
Gly
3.0%
3.9%
2.6%
5.5%
2.5%
1.0%
1.9%
3.2%
4.3%
5.0%
5.0%
7.2%
6.4%
3.7%
4.1%
9.4%
0.0%
6.2%
6.0%
Asp
2.0%
3.4%
1.7%
2.1%
4.2%
3.3%
4.2%
4.7%
4.3%
3.3%
1.1%
4.3%
2.3%
1.2%
6.8%
5.2%
3.9%
1.8%
3.8%
Glu
1.4%
3.7%
1.7%
2.0%
3.2%
1.2%
3.8%
3.6%
3.0%
2.9%
2.2%
4.4%
3.5%
3.2%
5.6%
5.1%
3.1%
1.5%
4.5%
Thr
3.1%
2.1%
2.6%
3.9%
3.1%
2.7%
1.3%
1.6%
3.6%
1.9%
0.8%
4.8%
2.8%
0.9%
2.6%
5.4%
2.5%
4.9%
5.1%
Ala
2.0%
1.3%
3.5%
3.4%
3.7%
1.3%
4.3%
4.3%
3.5%
2.7%
1.9%
5.3%
4.0%
2.3%
4.6%
4.5%
2.5%
4.2%
5.6%
Pro
0.6%
2.8%
1.8%
2.4%
1.9%
1.1%
1.4%
3.2%
4.2%
3.2%
3.0%
4.0%
2.3%
2.7%
3.8%
5.5%
2.1%
4.6%
5.3%
Lys
1.4%
3.2%
1.6%
2.8%
3.5%
3.0%
7.4% 10.2%
2.3%
3.9%
3.9%
3.4%
3.9%
4.9% 11.3% 8.9%
6.2%
5.1%
3.1%
Tyr
4.3%
5.5%
2.5%
6.2%
3.8%
2.9%
5.2%
5.7%
5.8%
2.5%
6.1%
4.3%
3.7%
1.8%
7.1%
9.2%
4.4%
4.9%
7.4%
xCys
4.2% 10.6% 5.1%
3.8%
5.1%
3.4%
3.9%
5.4%
6.5%
2.4%
3.4%
3.5%
10.5%
3.7%
8.1%
8.1%
5.2%
2.6%
6.1%
Met
5.7%
3.4%
1.4%
9.1%
7.7%
2.3%
2.1%
4.1%
4.1%
1.9%
1.8%
1.4%
3.3%
2.5%
4.0%
6.3%
1.9%
6.8%
7.2%
Val
10.0%
0.8%
9.4%
5.5% 13.3% 6.7%
9.4% 20.7% 13.9% 7.4%
4.3% 11.9%
2.5%
6.1% 12.8% 15.0% 6.5% 11.4% 12.2%
Ile
10.2%
0.5%
9.6%
8.3% 15.7% 8.6% 10.8% 22.4% 14.5% 8.8%
7.9% 13.7% 3.5%
8.0% 13.7% 14.1% 6.2% 11.8% 12.1%
Leu
1.8%
3.7%
2.4%
2.3%
1.8%
1.3%
2.7%
2.8%
5.2%
4.4%
3.4%
0.9%
3.1%
2.3%
6.2%
7.9%
1.8%
4.3%
7.5%
Phe
5.5%
5.2%
4.0%
5.2%
3.3%
2.5%
8.3%
7.0%
5.5%
6.1%
5.2%
5.5%
5.8%
4.4%
9.4%
8.5%
3.3%
3.7%
5.8%
Intermediate reproducibility data were obtained with robust statistics.
For each analyte/matrix pair, the intermediate reproducibility was compared with the corresponding reproducibility requirement. 32% of the
values are within the requirements (green) and 67% outside (red). 2% could not be attributed (blank values for taurine).
AMINO-01 (FEBRUARY 2017) - APPENDICES
FOR ERP USE ONLY
DO NOT DISTRIBUTE