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FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

1

Expert Review Panel for Infant Formula and Adult Nutrition

Evaluation of Method __CAR-03___________

Title: Carotenoids Lutein, beta-Carotene, Lycopene Single Laboratory Validation

Author:

Abbott Nutrition

Summary of Method:

This method extracts the carotenoids from the product using hexane/MtBE following saponification of

the sample. The sample is then dried and redissolved in a BHT/MtBE mixture prior to analysis using

HPLC-UV with C30 chromatography utilizing a gradient.

Method Scope/Applicability:

Method appears to be ideal for compounds of interest in SPIFAN initiative.

General comments about the method:

Simple method for the determination of the carotenoids using common laboratory supplies and

equipment. Could be easily set up anywhere in the world.

Method Clarity:

The Method was clear and easily understood.

Method Safety Concerns:

No major safety concerns about this method.

Pros/Strengths:

Method has the ability to determine lutein, lycopene and beta carotene in a single injection that is not

>45 minutes/injection

Common Lab instrumentation and supplies used for analysis