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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method __CAR-03___________
Title: Carotenoids Lutein, beta-Carotene, Lycopene Single Laboratory Validation
Author:
Abbott Nutrition
Summary of Method:
This method extracts the carotenoids from the product using hexane/MtBE following saponification of
the sample. The sample is then dried and redissolved in a BHT/MtBE mixture prior to analysis using
HPLC-UV with C30 chromatography utilizing a gradient.
Method Scope/Applicability:
Method appears to be ideal for compounds of interest in SPIFAN initiative.
General comments about the method:
Simple method for the determination of the carotenoids using common laboratory supplies and
equipment. Could be easily set up anywhere in the world.
Method Clarity:
The Method was clear and easily understood.
Method Safety Concerns:
No major safety concerns about this method.
Pros/Strengths:
•
Method has the ability to determine lutein, lycopene and beta carotene in a single injection that is not
>45 minutes/injection
•
Common Lab instrumentation and supplies used for analysis