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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
Cons
No ISTD
4 point curve used – minimum of 6 levels is recommended for SLV
Method performance requirements:
Lutein
Analytical Range:
Trans Lutein 10-250 µg/L reference standard
6.25 – 156.25 mcg/100g product
Linearity:
0.99991 and <1 % error
LOQ:
0.40 µg/100g RTF
Accuracy/Recovery:
90.3 -95.3 %
Precision (RSD
r
):
1-100 mcg/100g RTF - 1.89 – 7.32 %
14.88 % for child formula?
Intermediated Precision:
1-100 mcg/100g RTF - 1.93 – 8.06 %
13.99 % for Child formula?
Beta Carotene
Analytical Range:
Trans Lutein 25 - 500 µg/L reference standard
15.6 – 312.5 mcg/100g product
Linearity:
0.99993 and <1 % error
LOQ:
0.10 µg/100g RTF
Accuracy/Recovery:
89.3 -108.1 %
Precision (RSD
r
):
1-100 mcg/100g RTF - 1.81 – 6.77 %
Intermediated Precision:
1-100 mcg/100g RTF - 3.07 – 16.20 %
Lycopene
Analytical Range:
Trans Lutein 5-100 µg/L reference standard
3.125 – 62.5 mcg/100g product
Linearity:
0.9998 and <1.5 % error
LOQ:
0.30 µg/100g RTF
Accuracy/Recovery:
97.3 – 108.8 %
Precision (RSD
r
):
1-100 mcg/100g RTF - 3.01 – 6.37 %