AOAC SPIFAN ERP Reviewer Forms (March 16, 2017)

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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
• No major concerns over the chemicals involved
•
Cons/Weaknesses
• Spike recoveries on the lutein are all below 100%. Is the method under recovering lutein or is the
saponification too harsh on the sample destroying the lutein?
Supporting Data
• General Comment:
Positive that multiple analysts used in SLV
- Method Optimization:
 Were multiple column manufacturers used for C30
 Multiple Instruments?
• Performance Characteristics:
Analytical Range:
Method meets SMPR requirement
LOQ:
Method Meets SMPR requirement
Accuracy/Recovery:
Recoveries were acceptable except for Lutein. Though they meet the SMPR of 90-110%
requirement, the recoveries are all below 100%.
Precision (RSDr):