AOAC SPIFAN ERP Reviewer Forms (March 16, 2017)

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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
Method Safety Concerns: Safe handling of the chemicals involved listed
Pros/Strengths:
• Quite sensitive method
• The method lists steps to establish purity of standards.
Cons/Weaknesses
• Involves hot saponification
• Not able to distinctly separate previtamin D from vitamin D
Supporting Data
• General Comment: Required data are available
- Method Optimization: Data not provided
• Performance Characteristics:
Analytical Range:
The average range for vitamin D 2 concentration of 0.04–7.3 μg hg -1 RTF which extends
beyond limits of the range specified in the vitamin D SMPR.
The average range for vitamin D 3 0.06–11.3 μg hg-1 RTF which exceeds the range specified
in the vitamin D SMPR.
LOQ:
The LOQ for vitamin D2 = 0.016 μg hg-1.
The LOQ for vitamin D3 = 0.028 μg hg-1.
LOQ for both vitamin D2 and vitamin D3 are lower than those defined in the vitamin D
SMPR.