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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
3
SMPR requirement (11%) and are notable given the number of labs and diversity of matrices
included in the MLT.
Precision as Reproducibility (RSDR) – Reported values range from 6.4–12.7% and are within
SMPR requirement of 15%. Reproducibility for 9 of the matrices are <10%. This is also
notable. There also is no obvious performance trend vs Product type for either Repeatability
or Reproducibility.
MLT vitamin D
3
results returned for SRM 1849a were compared to the NIST certified value.
The overall mean reported by MLT of 10.2 mcg/100g and compares favorably to the NIST
certified value of 11.1 mcg/100g.
SLV Performance Summary:
Demonstrated method repeatability, accuracy, recovery and LOQ for both vitamin D
2
and D
3
presented during original SLV meet or exceed SMPR.
Analytical Range:
Vitamin D
2
: 0.04 – 7.3 mcg/100g RTF
Vitamin D
3
: 0.06 – 11.3 mcg/100g RTF
SMPR: 0.12 – 5.1 mcg/100g RTF
LOQ:
Vitamin D
2
: 0.016 mcg/100g RTF (≤0.120 mcg/100g RTF)
Vitamin D
3
: 0.028 mcg/100g RTF (≤0.120 mcg/100g RTF)
Accuracy/Recovery:
Vitamin D
2
: 97 – 101% (mean = 98%); (SMPR 90-110%)
Vitamin D
3
: 94 – 101% (mean = 98%); (SMPR 90-110%)
vs. NIST SRM - Vitamin D3: within certified range
Precision as Repeatability (RSD
r
):
Vitamin D
2
: NAP, none of SPIFAN matrices contain vitamin D
2
Vitamin D
3
: 1.5 – 5.3% (mean = 3.0%); (SMPR ≤11%)
Recommendation:
The data produced by the MLT meet SMPR requirements for both Reproducibility and
Repeatability.