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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
3
Accuracy/Recovery:
Recovery was evaluated using unfortified samples within the SPIFAN kit. Each matrix was
spiked at two levels: 50% (≈ 0.6 μg hg
-1
RTF) and 100% (≈ 1.1 μg hg
-1
RTF) of typical infant
formula concentrations. Recoveries measured for vitamin D
2
were between 97–101% and
for vitamin D
3
were between 94–101%, which are within the limits set in the SMPR of 90–
110%.
The method does not have any bias when its results compared against the values generated using
A std. AOAC reference method.
No bias between measured results and certified values for vitamin D3 in NIST SRM 1849a.
Precision (RSD
r
):
Repeatability for the method in SPIFAN kit samples ranged between 1.5–5.2% which
complies with the ≤ 11.0% limit set with the SMPR. The Intermediate precision ranged
between 3.1–7.9% with a mean value of 5.5%
Reproducibility (RSD
R
):
•
System suitability:
Recommendation:
The method may be considered by the ERP for the First Action Method.