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September 18–21, 2016
Sheraton Hotel, Dallas, Texas
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3:00 pm – 4:30 pm
Development of LC-MS/MS Methods and Reference
Materials for the Analysis of Food Allergens
Food allergen testing represents a difficult task for all
stakeholders. Traditional immunological-based methods
only detect certain epitopes of the allergenic proteins,
which might be subject to extensive modifications during
food production. This leads to highly diverse results in
allergen analysis, which may be overcome by liquid
chromatography-mass spectrometry/mass spectrometry
(LC-MS/MS) methods. Due to the high sensitivity,
selectivity, and accuracy of this approach, a wide range
of analytical methods using LC-MS/MS are rapidly being
developed. Problems of ELISA methods, such as cross-
reactivity of the antibody, matrix effects, and altered
protein epitopes can be overcome. It is also possible to
detect several food allergens in a single run.
Additionally, the lack of reference materials is a major
problem in allergen analysis and makes the quantification
of these analytes particularly challenging. All approaches
– including immunological methods, PCR and also LC-MS/
MS – require robust reference material to obtain reliable
and comparable results. Extensive input from the scientific
community is still required to solve this complex problem.
This session will discuss approaches of implementing
novel LC-MS/MS methods for the analysis of allergens in
food and food ingredients as well as the steps towards the
development of allergen reference materials.
CO-CHAIR:
Carmen Diaz-Amigo,
Consultant
CO-CHAIR:
Sabine Baumgartner,
IFA-Tulln
•
Sabine Baumgartner,
IFA-Tulln
Food Allergen Detection – Still a Challenge
•
Roland Poms,
MoniQA Association
Reference Materials for Food Allergen Analysis
•
Melanie Downs,
University of Nebraska
MS Analysis of Food Allergens: Challenges and Potential
Solutions
•
Bert Popping,
Merieux NutriSciences Corporation
LC-MS/MS: Do we Need Yet another Technology for
Allergen Testing?
Roundtable: Advancing Regulatory Science in Food
Testing—Sound Measurement, Analytical Science,
Sampling, and Quality Systems toward Food Safety
Regulation
The ultimate goal for a food safety regulatory agency is to
apply the best possible science for regulatory activities to
safeguard human and animal health. Sound measurement
science plays an important role in regulatory science and
fit-for-purpose analytical data is critical for regulatory
decisions. The focus of the proposed roundtable session
is how science based surveillance strategy, validated
methods, quality control and quality assurance programs
need to be integrated for the best practices in food/feed
testing laboratories. The roundtable session will utilize
short presentations and question-and-answer (Q&A)
interaction to engage speakers and the audience. Some
highlights of the round table session include: 1) the update
on the recently developed “Food/Feed Testing Laboratories
Best Practices Manual” by United States Food and Drug
Administration (USFDA) and multiple state participants;
2) the updates of the National Residue Program (NPR)
administrated by the United States Department of
Agriculture Food Safety and Inspection Service (USDA
FSIS), which is also the collaboration between FSIS,
FDA, and the Environment Protection Agency (EPA). The
session will also discuss product safety testing schemes,
laboratory quality systems, industry role in regulation and
co-regulation models. This roundtable session will show
that how science and regulation can be integrated for
a robust food protection system. The speaker/audience
discussion on real-life examples will be brain-storming for
the AOAC community to advance regulatory science.
CO-CHAIR:
Susie Dai,
Texas A&M University
CO-CHAIR:
Michael McLaughlin,
U.S. Food and Drug
Administration
CO-CHAIR:
Oliver Ou,
U.S. Department of Agriculture
•
Cynthia Mangione,
New York State Department of
Agriculture and Markets
Regulatory Science: Best Practices Manual for Food/
Feed Testing Laboratories
•
Oliver Ou,
U.S. Department of Agriculture
Protecting Public Health through Improving Test
Methodology and Modernizing the FSIS ALP Program
•
Ashli Brown,
Mississippi State University
Food/feed Safety from the State Level and beyond
•
Timothy Herrman,
Office of the Texas State Chemist
A Global View of Science Driven Approach for Food
Safety Regulation in East Africa
“Seed to Shelf” Analytical Roundtable for
Regulators and the Regulated: Analytical Methods,
Botanical Quality/Safety, FSMA and Dietary
Supplements cGMP
Current Good Manufacturing Practice regulations for
Dietary Supplements have now been in place since 2007,
and full compliance has been required since 2010. At
the same time food safety modernization act (FSMA)
from FDA was signed into a law in January 2011. Several
years of theoretical discussions are in the process of
becoming informed by actual laboratory practice and FDA
enforcement, and a number of FSMA provisions have
just gone into effect, with more to go into effect in the
summer of 2016. Two of the more interesting provisions
of FSMA are the requirement for laboratory accreditation
and the requirement that raw material suppliers create
and implement Hazard Analysis Critical Control Points
(HACCP) as part of their quality operations. This
session will engage the audience in an interactive panel
discussion with analysts from supplement company’s in-
house laboratories, small and large contract laboratories, an
innovative contract research organization, manufacturers