Page 131 - 2013 ISPAM Mid-Year Mtg Book

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Confirmation
Traditional confirmation
• Usually involves a combination of biochemical tests such as TSI,
LIA, urea in combination with serology (at least poly-O), with
additional serology for determining serovars. All equally valid?
What’s coming (or is here already)
• PCR confirmation from an isolate in lieu of biochemicals and poly-O
• Alternate systems for determining serovars – PCR, chemistry platforms
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Putting it all together
• Which steps of the method are most critical?
• Time differences?
• Temperature differences?
• pH adjustments (or not)?
• Do media formulations matter? Are some more inhibitory to
Salmonella?
• Differences in bias towards or selection for specific serovars?
• Other?
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