214 / 1195
42
10.5
Study Materials
1
2
BAX® System Real-Time PCR Assay for
Salmonella
Collaborative Study Materials
3
4
Materials supplied by DuPont
5
6
All BAX
®
System Real-Time PCR Assay for Salmonella detection kits (includes BAX® System
7
lysis buffer.–Two bottles of 12 mL each, BAX® System protease.–One vial of 400 µL, BAX®
8
System PCR tubes.–12 strips of 8 tubes containing one PCR tablet per tube.), BAX® System
9
Q7 cycler/detector with computer workstation, BAX® System application software and
10
BAX
®
MP Media
11
12
Materials provided by Q Laboratories, Inc., Coordinating Laboratory
13
14
1.
Standard Methods Agar for APC of test matrices
15
16
Enrichment
17
2.
Universal Pre-enrichment broth
18
3.
BPW
19
4.
RV-R10 broth for USDA reference method
20
5.
Individual Ingredients to prepare FDA RV
21
6.
TT Broth (Hajna formulation) for USDA reference method
22
7.
TT Broth for FDA reference method
23
24
Selective agar
25
8.
Hektoen Agar
26
9.
XLD Agar
27
10.
Bismuth Sulfite
28
11.
XLT4 Agar
29
12.
Brilliant green sulfa agar
30
31
Confirmation
32
13.
TSI
33
14.
LIA
34
15.
BHI
35
16.
API 20E or VITEK GN cards
36
17.
O and H serotyping supplies
37
38
Additional Supplies and Reagents required but not provided
39
18.
Pipettes.–For transferring reagents; two adjustable mechanical pipettes covering 20-
40
200
µ
L and 5-50
µ
L; one repeating pipette; and one multi-channel pipette covering 8
41
channels and 5-50
µ
L. Pipettes should be calibrated to deliver required volumes within
42
10%.
43
Collaborative Study Approved Protocol
Expert Review Panel Use Only