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43
19.
Pipette tips with barriers.–0.5-250
µ
L, 0.5-100
µ
L extended barrier; 5 mL repeater
1
pipette tips.
2
20.
PCR tube holders.–For transferring a rack of tubes from the cooling block to the
3
cycler/detector.
4
21.
Incubators.–For maintaining enrichment media at 35
±
1
°
C and 39-42
°
C.
5
22.
Cluster tubes and racks. –For lysis.
6
23.
Capping/de-capping tools.–For removing and sealing cluster tube caps and PCR tube
7
caps without jarring the contents.
8
24.
Thermal block(s) with inserts.–For maintaining lysis tubes at 37 ± 2°C, 95 ± 3°C, and
9
4 ± 3°C.
10
25. Water bath- Capable of maintaining 42 ± 1°C for secondary selective enrichments.
11
26.
Refrigerator – capable of maintaining 2-8°C, for storing the BAX® System Real-Time PCR
12
Assay for
Salmonella
test kits
13
27.
Stomacher®.–Seward model 400 or equivalent.
14
28.
Stomacher® type bags or equivalent.
15
29.
Blender.–High speed (16,000 – 18,000 rpm) with sterile receptacle.
16
17
*
Note: Please ensure that ample incubator space is available to accommodate the larger test
18
portions. We estimate that each lab will need approximately 54 cubic feet (3 large incubators
19
or an incubator room) to facilitate the testing of the 325 gram test portions. Overfilling the
20
incubator with too many test samples may compromise the integrity of the results.
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
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Collaborative Study Approved Protocol
Expert Review Panel Use Only