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AOAC INTERNATIONAL
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2. METHOD MODIFICATION AUTHORS
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F. Morgan Wallace, Stephen Varkey, Daniel DeMarco, George Tice, Bridget Andaloro, Dawn Fallon,
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Jeff Rohrbeck, Eugene Davis, Monica Tadler, Steven Hoelzer
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DuPont Qualicon
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3. SUBMITTING LABORATORY
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DuPont Qualicon, P.O. Box 80400, Route 141 and Henry Clay Road, Wilmington, DE 19880
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Phone 800-863-6842 or 302-695-5300 Fax 302-695-5301
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4. SCOPE OF METHOD
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1. Target organisms –
Salmonella
species.
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2. Matrices – Specific matrices tested during the modification studies included ground beef,
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cream cheese and dry pet food.
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3. Performance claims – Sensitivity and specificity comparable to the appropriate reference
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culture methods and equivalent or better than the unmodified method.
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5. PRINCIPLE
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PCR amplification
- The BAX® System uses the Polymerase Chain Reaction (PCR) to amplify a
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specific fragment of bacterial DNA, which is stable and unaffected by growth environment. The
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fragment is a genetic sequence that is unique to the genus
Salmonella
, thus providing a highly
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reliable indicator that the organism is present. The BAX® System simplifies the PCR process by
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combining the requisite primers, polymerase and nucleotides into a stable, dry, manufactured tablet
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already packaged inside the PCR tubes. These tubes remain sealed after amplification, significantly
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Dupont BAX Salmonella PTM Report
Modification Approved 2012 / PTM Certification No. 081201
For Expert Review Panel Use Only
Do Not Distribute