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AOAC INTERNATIONAL
8. ADDITIONAL REAGENTS
109
Lysis reagent
– Using test kit reagents, pipette 150
µ
L of protease into one 12-mL bottle of lysis
110
buffer. Label bottle with the date prepared. Reagent will remain stable for up to two weeks if
111
stored at 2-8ºC.
112
113
9. APPARATUS
114
Incubators
– For maintaining media at 35
±
1ºC and 39-42ºC.
115
Stomacher
– Seward model 400 or equivalent for mixing the sponge sample with enrichment
116
media.
117
BAX
®
System apparatus
118
BAX® System Q7 cycler/detector with computer workstation
119
BAX® System application software
120
Cluster tubes with caps and racks for lysis
121
Capping/de-capping tools – for removing and sealing cluster tube caps and PCR
122
tube caps without jarring the contents
123
Heating and cooling blocks with inserts – for maintaining lysis tubes at 37±2ºC,
124
95±3ºC, and 2-8°C.
125
Pipettes – for transferring reagents; two adjustable mechanical pipettes covering 20-
126
200
µ
L and 5-50
µ
L; one repeating pipette; and one multi-channel pipette covering 8
127
channels and 5-50
µ
L. Pipettes should be calibrated to deliver required volumes
128
within 10%.
129
Pipette tips with barriers: 0.5-250
µ
L, 0.5-100
µ
L extended barrier; 5 mL repeater
130
pipette tips
131
Dupont BAX Salmonella PTM Report
Modification Approved 2012 / PTM Certification No. 081201
For Expert Review Panel Use Only
Do Not Distribute