225 / 1195
AOAC INTERNATIONAL
10. Rappaport-Vassiliadis Soya Peptone (RVS), Selenite Cystine (SC), Tetrathionate-Hajna (TT-
157
Hajna) and Tetrathionate (TT) broths and Xylose Lysine Desoxycholate (XLD), Hektoen Enteric
158
(HE), Brilliant Green Sulfa (BGS) and Bismuth Sulfite (BS) agars and other appropriate
159
confirmatory media for reference method and alternate culture method confirmation.
160
161
12. SAFETY PRECAUTIONS
162
Kits
— Reagents used in the BAX® System
should pose no hazards when used as directed.
163
Dispose of lysate, PCR mixture and other waste according to your site practices.
164
Cycler/detector
— Only qualified laboratory personnel should operate the cycler/detector. Do not
165
attempt to repair the instrument. Live power may still be available inside the unit even when a
166
fuse has blown or been removed. Refer to the BAX® System User Guide for maintenance
167
procedures when cleaning the unit or changing a fuse. The heating block can become hot enough
168
during normal operation to cause burns or cause liquids to boil. Wear safety glasses or other eye
169
protection at all times during operation.
170
Enrichment Broths
— All enrichment broths may contain varying pathogens whether they contain
171
Salmonella
or not and thus should be sterilized and disposed of using proper procedures
172
following any culture-based confirmatory steps.
173
174
13. GENERAL PREPARATION / SAMPLE PREPARATION AND
175
RECOVERY
176
Sample Preparation
177
Ground beef, cream cheese and dry pet food samples were prepared for testing with either the
178
BAX® System method or the appropriate reference method as summarized in Table 1. Each sample
179
matrix was inoculated with a distinct
Salmonella
strain selected from the DuPont Qualicon Culture
180
Collection. A pure culture of each strain was inoculated into BHI broth and grown at 37°C overnight to
181
Dupont BAX Salmonella PTM Report
Modification Approved 2012 / PTM Certification No. 081201
For Expert Review Panel Use Only
Do Not Distribute