AOAC RI ERP Final Action Recommendations - BOOK

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AOAC INTERNATIONAL
PCR tube holders – for transferring a rack of tubes from the cooling block to the 132
cycler/detector 133
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10. STANDARD REFERENCE CULTURES 135
DuPont Qualicon Culture Collection (DD) - proprietary 136
American Type Culture Collection (ATCC) - 137
American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA 138
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11. MEDIA AND KIT COMPONENTS 142
Items 1-4 are available as part of the BAX® System Real-Time PCR Assay for Salmonella. 143
1. BAX® System PCR tubes (12 strips of 8 tubes containing one PCR tablet per tube) 144
2. BAX® System lysis buffer (two bottles of 12 mL each); 145
3. BAX® System protease (1 vial of 400 µL) 146
4. Flat optical caps (12 strips of 8 caps) 147
5. Lactose broth – Dissolve 3 g of beef extract, 5 g of peptone and 5 g of lactose into 1 L of 148
distilled water. Dispense into containers and autoclave 15 minutes at 121ºC. Final pH is 6.9+/-0.2 149
6. BAX® System MP media – DuPont Qualicon catalog D12404925 (bulk powder), D12745725 150
(StatMedia) 151
7. Modified Tryptic Soy Broth containing glucose and 2 mg/L novobiocin (TSB + n), Difco 152
catalog number 211825 or equivalent, BD, Franklin Lakes, NJ, 07417, USA 153
8. Buffered Peptone Water (BPW) – Oxoid CM 0509 or equivalent, Oxoid, Hampshire, United 154
Kingdom, RG24 8PW 155
9. Brain Heart Infusion (BHI) broth – Oxoid CM 1032 or equivalent. 156
Dupont BAX Salmonella PTM Report
Modification Approved 2012 / PTM Certification No. 081201
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