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Evaluation of the 3M
™
Molecular Detection Assay
Salmonella
for the
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Detection of
Salmonella
in Select Foods
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3
AOAC Research Institute
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Performance Tested Method
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Abstract
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The 3M™ Molecular Detection Assay
Salmonella
is used with the 3M™ Molecular Detection System for
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the rapid and specific detection of
Salmonella
in enriched food, feed and environmental samples. Six
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matrices – raw ground beef, cooked breaded chicken, raw shrimp, bagged spinach, wet pet food, and
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pasteurized liquid whole egg – were inoculated with
Salmonella
. Twenty (20) replicates of each matrix
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were analyzed at one inoculum level: 0.2-2 CFU/test portion. Five (5) control replicates were also
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analyzed at 0 CFU/test portion (un-inoculated). The 3M Molecular Detection Assay
Salmonella
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method was compared to the FDA/ BAM Chapter 5
Salmonella
reference method
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for raw shrimp,
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bagged spinach, and wet pet food, and to the USDA/FSIS MLG 4.05
Isolation and Identification of
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Salmonella from Meat, Poultry, Pasteurized Egg, and Catfish Products
reference method
2
for raw
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ground beef, cooked breaded chicken and pasteurized liquid whole egg. There were no statistically
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significant differences in the number of positive samples detected by the 3M Molecular Detection
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Assay
Salmonella
method and the reference methods for all 6 matrices analyzed. Lot-to-lot assay
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variability, shelf-life stability, and three assay ruggedness parameters (lysis time and volume variation,
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and enrichment volume variation) were evaluated for the 3M Molecular Detection Assay
Salmonella
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and found no unexpected results. The 3M Molecular Detection Assay
Salmonella
method
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demonstrated reliability as a rapid qualitative method for the detection of
Salmonella
in select foods.
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Method Author
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DeAnn L. Benesh
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3M Food Safety Department
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3M Center - Bldg 260-6B-01
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St. Paul, MN 55144-1000
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Submitting Company
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3M Company
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Food Safety Department
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3M Center,
Bldg. 275-5W-05
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St. Paul, MN 55144-1000
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Independent Laboratory
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Food Safety Net Services
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199 W. Rhapsody
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San Antonio, TX 78216
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Reviewers of Final Submission
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Thomas Hammack
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Microbial Methods Development Branch
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Division of Microbiology
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