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Safety Precautions
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The 3M Molecular Detection Assay
Salmonella
is intended for use in a laboratory environment by
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professionals trained in laboratory techniques. The user should read, understand and follow all safety
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information in the instructions for the 3M Molecular Detection System and the 3M Molecular
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Detection Assay
Salmonella
and retain the safety instructions for future reference. Follow all
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instructions carefully. Failure to do so may lead to inaccurate results.
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The 3M™ Molecular Detection Instrument is intended for use with samples that have undergone heat
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treatment during the assay lysis step, which is designed to destroy organisms present in the sample.
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Samples that have not been properly heat treated during the assay lysis step may be considered a
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potential biohazard and should NOT be inserted into the 3M Molecular Detection Instrument.
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Store the 3M Molecular Detection Assay
Salmonella
at 2-8°C. Do not freeze. Keep kit away from light
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during storage. After opening the kit, check that the foil pouch is undamaged. If the pouch is damaged,
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do not use. After opening, unused reagent tubes should always be stored in the re
‑
sealable pouch
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with the desiccant inside to maintain stability of the lyophilized reagents. Store resealed pouches at 2-
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8°C for no longer than 1 month. Do not use 3M Molecular Detection Assay
Salmonella
past the
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expiration date. Expiration date and lot number are noted on the outside label of the box.
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After use, the enrichment medium and the 3M Molecular Detection Assay
Salmonella
tubes can
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potentially contain pathogenic materials. When testing is complete, follow current industry standards
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for the disposal of contaminated waste. Consult the Material Safety Data Sheet for additional
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information and local regulations for disposal.
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General Preparation
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Use proper aseptic technique. Use proper precautions for biosafety level 2 microorganisms.
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Periodically laboratory benches and equipment (pipettes, cap/decap tools, etc…) should be
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decontaminated with a 1-5% (v:v in water) household bleach solution or DNA removal solution.
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Sample Preparation
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Table 1 presents guidance for the enrichment of food and feed samples of 25 g and 375 g at a 1:10
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dilution. It is the user’s responsibility to validate alternate sampling protocols or dilution ratios to
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ensure this test method meets the user’s criteria.
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1.
Pre-warm 3M BPW ISO enrichment medium to 37 ±1°C.
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2.
Aseptically combine the enrichment medium and sample. Homogenize thoroughly for 2 minutes
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Incubate at 37 ±1°C.
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3.
For all meat and highly particulate samples, the use of filter bags is recommended.
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In an AOAC Research Institute Performance Tested Method study, the 3M Molecular Detection Assay
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Salmonella
was found to be an effective method for the detection of Salmonella in the following matrices:
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Sample Matrix
Sample Size
Enrichment
Broth Volume
(mL)
Enrichment
Time (hr)
Raw ground beef [27% fat]
8-18
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