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AOACRI

Certificate

No.

XXXXXX

Raw shrimp

25 g

225

18-24

Bagged spinach

Pasteurized liquid whole egg

100 g

900

Cooked breaded chicken

325 g

2925

Wet pet food

[Dog – beef cuts in gravy,

canned]

375 g

3375

For other

matrices

Foods

25 g

225

Environmental

1 sponge

225

1

Preparation of the 3M™ Molecular Detection Speed Loader Tray

2

1.

Wet a cloth with a 1-5% (v:v in water) household bleach solution and wipe the 3M™ Molecular

3

Detection Speed Loader Tray.

4

2.

Rinse the 3M Molecular Detection Speed Loader Tray with water.

5

3.

Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.

6

4.

Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.

7

8

Preparation of the 3M™ Molecular Detection Chill Block Insert

9

Before using the 3M™ Molecular Detection Chill Block Insert, ensure it has been stored on the 3M™

10

Molecular Detection Chill Block Tray in the freezer (-10 to -20°C) for a minimum of 2 hours before use.

11

When removing the 3M Molecular Detection Chill Block Insert from the freezer for use, remove it and

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the 3M Molecular Detection Chill Block Tray together. Use the 3M Molecular Detection Chill Block

13

Insert /3M Molecular Detection Chill Block Tray within 20 minutes.

14

15

Preparation of the 3M™ Molecular Detection Heat Block Insert

16

Place the 3M™ Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on the

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dry block heater unit and set the temperature to 100 ±1°C.

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NOTE:

Depending on the heater unit, allow approximately 30-50 minutes for the 3M Molecular Detection Heat

20

Block Insert to reach temperature. Using a calibrated thermometer, verify that the 3M Molecular Detection Heat

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Block Insert is at 100 ±1°C.

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Preparation of the 3MMolecular Detection Instrument

24

1.

Launch the 3M™ Molecular Detection Software and log in.

25

2.

Turn on the 3M Molecular Detection Instrument.

26

3.

Create or edit a run with data for each sample. Refer to the 3M Molecular Detection System User

27

6