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AOAC EXPERT REVIEW PANEL FOR MICROBIOLOGY FOR FOODS AND ENVIRONMENTAL SURFACES

AOAC Expert Review Panel Final Action Feedback

Table 4. BAX® System vs. Reference Method Results – Meat and SeafoodRevised Data

Sample

Type

Media

MPN

a

Test

Portions

BAX® Method

Reference Method

dPOD

C

e

95% CI

f

X

b

POD

C

c

95% CI

f

X

b

POD

R

d

95% CI

f

Raw

ground

beef with

soy (25 g)

BPW

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45,

0.45)

0.94 (0.68,

1.3)

60

36 0.60

(0.47,

0.71)

36

0.60

(0.47,

0.71)

0

(-0.049,

0.049)

8.6 (6.2,

12)

5

5

1.0

(0.57,

1.0)

5

1.0

(0.57,

1.0)

0

(-0.45,

0.45)

Raw

ground

beef with

soy (325 g)

mTSB+caa+n

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.43,

0.43)

0.94 (0.68,

1.3)

60

39 0.65

(0.52,

0.76)

36

0.60

(0.47,

0.71)

0.05

(-0.12,

0.22)

8.6 (6.2,

12)

5

5

1.0

(0.57,

1.0)

5

1.0

(0.57,

1.0)

0

(-0.43,

0.43)

a

MPN = Most Probable Number based on the POD of reference method test portions

using the AOAC MPN calculator (with 95% confidence interval)

b

X = Number of positive test portions

c

POD

C

=

Confirmed candidate method positive outcomes divided by the total number

of trials

d

POD

R

= Confirmed reference method positive outcomes divided by the total number

of trials

e

dPOD

C

= Difference between the candidate method and reference method POD values

f

95% CI = If the confidence interval of a dPOD does not contain zero, then the

difference is statistically significant at the 5% level

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