56 / 1195
AOAC EXPERT REVIEW PANEL FOR MICROBIOLOGY FOR FOODS AND ENVIRONMENTAL SURFACES
AOAC Expert Review Panel Final Action Feedback
Figure 2. Tablets hydrated and loaded into the instrument 10 min after hydration.
II.
The following change is requested during evaluation by the ERP for final action status:
After several incidents of very low target cell density enrichments being incorrectly called
negative by the software, a change will be made in the BAX® software algorithm for this test
kit to correct these erroneous calls. As a result of this change, two of 1938well calls
associated with AOAC validation studies (both from the pre-collaborative study, one from
the 25 g ground beef matrix and one from the 25 g ground turkey matrix) changed from
correct negative calls to incorrect positive calls. The two amplification plots shown in
Figures 3 and 4 are the PCR results from the samples for which algorithm calls changed with
the new algorithm.
Corrected statistical tables are presented for the two matrices in question. Tables 1 and 2
and 5 and 6 show the original data, while Tables 3 and 4 and 7 and 8 show the data when
the results are analyzed with the new BAX® RT
Salmonella
algorithm. In both cases, the
Page
5
of
13