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Table 4.

Comparison of LPT and USDA/FSIS MLG Methods for Detection of

Listeria

in Food – 125g

a

American Type Culture Collection

b

X

2

is defined by Mantel-Haenszel as (n-1)(AF-(B+C+D)E)

2

/(A+B+C+D)(A+E)B+C+D+F)(E+F) where n+A+B+C+D+E+F. A X

2

value greater than 3.84 indicates

significance at

p

>0.05.

Matrix

Test

Portion

Size

APC

Results

Contaminatio

n Level

MPN/125g

Total

Sample

s

VIDAS LPT

Reference

Method

Chi

Sq.

c

Positive Confirme

d

Deli ham

L.monocytogenes

ATCC

a

7644

125g

60 cfu/g

Uninoculated

NA

5

0

0

0

-

Low

1.31

20

16

16

15

0.14

Ground beef

L.monocytogenes

ATCC 13932

125g

120

cfu/g

Uninoculated

NA

5

0

0

0

-

Low

0.94

20

7

7

7

0

Deli turkey

L.monocytogenes

ATCC 51776

125g

<10

cfu/g

Uninoculated

NA

5

0

0

0

-

Low

0.70

20

11

11

12

0.10