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VIDAS LPT - Summary 2012
EUROFINS IPL Nord SAS
–
1 rue du Professeur Calmette
–
59046 LILLE cedex
NORME ISO 11290-1 and amendment A1
YES
YES
YES
NO
NO
Incubation for 24 h at 37°C ± 1°C
Incubate for 24 h
at 37°C ± 1°C
Prepare test sample (x g ou x mL)
Dilute to 1:10 in Fraser ½ broth
Isolate onto
Listeria
agar
OAA and Palcam
Transfer 0,1 mL of Fraser ½
enrichment in 10 mL Fraser 1
Plating on selective
Listeria
agar
OAA and Palcam
Presence of typical
colonies
Re-incubation 24 h à 37°C
±1°C
Presence of typical
colonies
Confirmation
L. monocytogenes
Response :
Presence of
Listeria monocytogenes
in x g or x mL
Incubation for 24 h at 30°C ± 1°C
Incubation for 48 h at 37°C ± 1°C
NO
Response :
Absence of
Listeria monocytogenes
in x g or x mL