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VIDAS LPT - Summary 2012
EUROFINS IPL Nord SAS
–
1 rue du Professeur Calmette
–
59046 LILLE cedex
VIDAS
®
UP
Listeria
(VIDAS
®
LPT) METHOD
Specific protocole for surface samples
Presence of
typical colonies
VIDAS
®
LPT
positive
Incubation for
24 ± 3 h at 37°C ±1°C
And additional 24h in absence of typical
colonies
NO
YES
YES
NO
NO
YES
Enrichment
-
Ecouvillon + 10 mL LPT broth
-
Sponge + 100 mL LPT broth
With preheated at room temperature LPT broth (18-25°C)
Incubation for 22 h to 30 h at 30°C ±1°C
Response :
Absence of
Listeria
spp
.
in
the sample
Isolate onto selective
Listeria
agar
Palcam and OAA
Confirmations
After homogenizing of
enrichment, transfer 1-2 mL
in a tube, seal and heat at
95-100°C for 5 min
Store LPT enrichment at 2-8°C
for subsequent confirmation of
positive tests.
Response :
Presence of
Listeria
spp
.
in
the sample
Pipet 0.5 mL in a VIDAS LPT strip