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VIDAS LPT - Summary 2012
EUROFINS IPL Nord SAS
–
1 rue du Professeur Calmette
–
59046 LILLE cedex
VIDAS
®
UP
Listeria
(VIDAS LPT) METHOD
General protocol
Presence of
typical colonies
Incubation for
24 ± 3 h at 37°C ±1°C
And additional 24h in absence of typical
colonies
NO
YES
YES
NO
NO
YES
Enrichment
Test portion (x g or x mL)
in 9x mL LPT broth
preheated at room temperature (18-25°C)
Incubation for 26 h to 30 h at 30°C ±1°C
Pipet 0.5 mL in a VIDAS LPT strip
Response:
Absence of
Listeria
spp
.
in x g
or x mL
Isolate onto selective
Listeria
agar
Palcam and OAA
VIDAS
®
LPT
positive
Confirmations
After homogenizing of
enrichment, transfer 1-2 mL
in a tube, seal and heat at
95-100°C for 5 min
Store LPT enrichment at 2-8°C
for subsequent confirmation of
positive tests.
Response:
Presence de
Listeria
spp
.
in x g
or x mL