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Silliker
Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis
Silliker Laboratories, Chemistry Research Center, Date: 1/30/15
10
evaluated range of 5 to 250 ng/mL. Examples of the calibration curve of vitamin K1 peak area and also
peak area as a ratio of peak area of internal std. and also for trans vitamin K1 are provided in Figures
2a, 2b and 2c, respectively. Calibration curve of K2-7 and K2-4 are provided in Figures 3 and 4
respectively.
Figure 2a: One of the example of the Calibration curve (linear regression) for vitamin K1, vitamin K1
conc (ng/mL) (X axis) and peak area for respective standard (YAxis) and their linear regression
relationship.
Figure 2b One of the example of the Calibration curve (linear regression) for vitamin K1, vitamin K1
conc (ng/mL) (X axis) and peak area ratio of vitamin K1 and internal std for respective standard (Y
Axis) and their linear regression relationship.
y = 2233.7x - 1767.6
R² = 0.9995
0
200000
400000
600000
800000
1000000
1200000
0
100
200
300
400
500
600
Vit K1
y = 0.161x - 0.133
R² = 0.9996
0
5
10
15
20
25
30
35
40
45
0
50
100
150
200
250
300
Vit K1 (ratio of int std)
VitK-01 w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE