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Silliker
Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis
Silliker Laboratories, Chemistry Research Center, Date: 1/30/15
16
Table 5a: Analysis of different SPIFAN samples in replicates on
multiple days - Day 1 Results
Day 1 of Multiple Day Replicate analysis
Sample ID
Paramtrs
Trans
Cis
Total (trans +
cis)
mcg/100g of RF sample as is or a powder sample
reconstituted in to a liquid
SPIFAN
Sample #6
Mean
10.5
1.5
12.0
Stdev
0.2
0.1
0.3
% RSD
2.0
6.3
2.4
n
3
3
3
SPIFAN
Sample #7
Mean
2.4
1.2
3.6
Stdev
0.1
0.1
0.2
% RSD
6.0
6.8
6.2
n
3
3
3
SPIFAN
Sample #8
Mean
5.7
1.1
6.8
Stdev
0.6
0.0
0.6
% RSD
10.9
3.4
9.5
n
3
3
3
SPIFAN
Sample #9
Mean
7.4
1.1
8.5
Stdev
0.1
0.0
0.1
% RSD
1.1
3.0
1.0
n
3
3
3
SPIFAN
Sample
#10
Mean
2.7
0.3
3.0
Stdev
0.2
0.0
0.2
% RSD
6.8
12.8
7.2
n
3
3
3
SPIFAN
Sample
#11
Mean
2.3
0.4
2.7
Stdev
0.1
0.0
0.1
% RSD
4.2
12.9
5.3
n
3
3
3
SPIFAN
Sample
#12
Mean
8.2
0.9
9.0
Stdev
0.3
0.1
0.3
% RSD
3.8
7.7
3.2
n
3
3
3
SPIFAN
Sample
#13
Mean
5.4
1.1
6.5
Stdev
0.6
0.1
0.7
% RSD
11.0
13.1
11.4
n
3
3
3
SPIFAN
Sample
#14
Mean
5.6
1.0
6.6
Stdev
0.1
0.0
0.0
% RSD
1.0
3.1
0.3
n
2
2
2
VitK-01 w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE