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Silliker
Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis
Silliker Laboratories, Chemistry Research Center, Date: 1/30/15
15
Table 5 : Reconstitution of the powder samples (for evaluation of the method
repeatability/precision/spike recovery evaluation) before the analysis
Sample #
Sample Name
Lot#ID
Powder for
Reconstitution
(g)
Water
Added (g)
1*
Child Formula Powder
00411RF00
12.00093
100
2*
Infant Elemental Powder
00402RF00
12.0037
100
3*
Adult Nutritional RTF, High Protein
00415RF00
4*
Adult Nutritional RTF, High Fat
00407RF00
5*
Infant Formula RTF, Milk Based
SPIFAN
Blank
Milk Form
6
SRM 1849a
CLC10-b
12.0364
100
7
Adult Nutritional Powder Milk Protein
Based
11750017V3
12.0203
100
8
Infant Formula Powder Partially
Hydrolyzed Milk based
1172572116
12.0076
100
9
Infant Formula Powder Partially
Hydrolyzed Soy Based
117257651Z
12.0867
100
10
Adult Nutritional Powder Low Fat
00394RF00
12.0461
100
11
Child Formula Powder
00412RF00
12.0862
100
12
Infant Elemental Powder
00403RF00
12.00076
100
13
Infant Formula Powder Milk Based
D04HTCVV09
12.0036
100
14
Infant Formula Powder Soy Based
E29JVL05
12.0504
100
15
Infant Formula RTF Milk Based
SPIFAN
Control
Milk Form
16
Adult Nutritional RTF High Protein
00414RF00
17
Adult Nutritional RTF High Fat
00406RF00
* Samples used for spike recovery evaluation.
Averages and Precision of within day Replicates analysis of SPIFAN sample tested for separate
three days:
Beverages prepared from the respective powdered samples and the RTF samples (as is) were analyzed
for all the targeted vitamin K compounds. The Vitamin K compounds in each powder sample was
analyzed in the reconstituted liquid sample. The Results of the averages and repeatability/precision
(%RSD
r
) for each of the sample for every day of the analysis are calculated. The results are presented
in the following Table 5 a-c.
VitK-01 w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE