118 / 164
Silliker
Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis
Silliker Laboratories, Chemistry Research Center, Date: 1/30/15
12
Figure 4: One of the example of Calibration curve (linear regression) for vitamin K2-4. Vitamin K2-4
conc (ng/mL) (X axis) and peak area for respective concentration (YAxis) and their linear regression
relationship.
Selected calibration parameters for the targeted vitamin K compounds based on one of the calibration
curve are summarized in Table 3.
Table 3: Evaluation of Response Linearity against concentration of vitamin K compounds
Vitamin K1
Trans-vitamin K1
Vitamin K2-7
Vitamin K2-4
Slope
2234
1864
519
855
R^2
0.9995
0.9994
0.9991
0.9995
The calibration values based on the presented example calibration curve ( conc. Vs peak area) for
different vitamin K compounds are listed in the above Table 3. All of the analytes provided a good
linear response against their respective concentrations.
The calibration of vitamin K1 against ratio of peak area of vit K1 and internal std. is provided in Figure
5.
y = 854.75x - 2476.9
R² = 0.9995
0
50000
100000
150000
200000
250000
300000
350000
400000
450000
0
100
200
300
400
500
600
Vit K2-4
VitK-01 w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE