119 / 164
Silliker
Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis
Silliker Laboratories, Chemistry Research Center, Date: 1/30/15
13
Figure 5: One of the example of Calibration curve (linear regression) for vitamin K1. Vitamin K1 conc
(ng/mL) (X axis) and ratio of peak area for respective concentration/peak area of internal std. (YAxis)
and their linear regression relationship.
The relationship between the Ratio of vitamin K1 peak area and internal std for respective vitamin K1
concentration was found to be linear , r2 = 0.9996.
Method Validation Results:
The Evaluation of Accuracy and Precision of the Method by Analysis of Reference Materials :
The method accuracy was evaluated by analysis of a Reference material, NIST SRM 1849a (a milk
based infant/adult nutritional formula) with a Certified value for vitamin K1 of 1.06 mcg/g + 0.17 .
The NIST SRM was analyzed on five different days. The Results are presented in Table 4.
y = 0.161x - 0.133
R² = 0.9996
0
5
10
15
20
25
30
35
40
45
0
50
100
150
200
250
300
Vit K1 (with ratio of int std)
VitK-01 w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE