Program Book - page 66

66 Biophysical Society 58th Annual Meeting, San Francisco, California
Monday, February 17
7:30 am–8:30 am, Room 302
Graduate Student Breakfast
This breakfast presents an opportunity for graduate student members of
the Society to meet and discuss the issues they face in their current career
stage. Members of the Early Careers Committee will be there to talk about
grant activities specific to graduate students. They will also be available
to answer questions about how the Committee serves graduate students
in the biophysical community, and to recruit new Committee members.
Limited to the first 100 attendees.
7:30 am–5:00 pm, North Lobby
Registration/Exhibitor Registration
7:30 am–10:00 pm, Room 112
Family Room
8:00 am–8:45 am, Room 123
Exhibitor Presentation
FEI Company
Making Correlative Experiments Easier
Fluorescence microscopy excels at labeling components of the cellular
machinery with unmatched sensitivity and specificity; however, it lacks
any contextual information. Providing full morphological information
at the ultra-structural level is the strength of electron microscopy. If the
very same sample is imaged by fluorescence and electron microscopy it is
possible to merge dynamics, label specificity and nanometer resolution.
Although a powerful approach, it is challenging and low-throughput.
FEI has recently introduced new solutions to overcome these experimental
hurdles: CorrSight, a dedicated light microscopy system offering CLEM-
specific functionality and automation of important workflow steps; MAPS, a
software tool bridging the modalities to increase ease of use; and iCorr, a light
microscope module integrated into the Tecnai family of transmission electron
microscopes. These tools address different correlative workflows helping to op-
timize efficiency and data quality across the full range of CLEM experiments.
CorrSight is an innovative light microscope providing unprecedent solu-
tions to optimal sample support for different workflows in correlative light
and electron microscopy. One of its strengths is the possibility to perform
live cell imaging, event-triggered fixation and subsequent processing of the
sample for electron microscopy. On top of it a dedicated cryo stage allows
contamination-free imaging of vitrified samples with the highest resolution.
MAPS also allows for correlation of light microscopy data captured on any
light microscope with EM data acquisition on the full range of FEI SEMs
/ SDBs. To allow utmost flexibility in the choice of the light microscope,
there is absolutely no dependence on any special hardware – correlation is
carried out only on image data. Thus, existing or specialized light micros-
copy setups can be easily used for CLEM experiments and correlation can
be carried out on any feature visible in both modalities. When coupled
with CorrSight the correlation is possible without manual intervention.
In order to perform correlative experiments between LM and TEM imag-
ing FEI has developed an integrated light and electron microscope: iCorr.
This tool provides fast and effortless navigation for correlative experiments.
Presenters:
Alex de Marco, Product Marketing Manager, FEI Munich GmbH
Gregor Heiss, Product Marketing Engineer, FEI Munich GmbH
Liesbeth Hekking, Applications Development Engineer, FEI Company
Matthias Langhorst, Segment Director Cell Biology Solutions, FEI Company
8:00 am–5:30 pm, Room 300
Career Center
8:00 am–6:00 pm, Marriott Marquis, Pacific H, I, J
Child Care
8:00 am–6:00 pm, Rotunda, 300 Level
Undergraduate Student Lounge
This special space is reserved for undergraduate meeting attendees looking
for a place to relax or catch up on coursework they may be missing while
at the Annual Meeting. Members of the Education Committee, which
sponsors this lounge, will stop by to answer questions student attendees
may have about career paths and opportunities.
8:00 am–10:00 pm, Hall D
Poster Viewing
8:15 am–10:15 am, Room 134
Symposium
Molecular Basis for Regulation
of Ca2+ Channels
Co-Chairs
Amy Lee, University of Iowa
Stephen Long, Memorial Sloan-Kettering Cancer Center
1111-Symp 8:15 am
ORIGIN AND MECHANISM OF MITOCHONDRIAL FLASHES.
Heping Cheng, Xianhua Wang, Qi Ma, Wang Wang
1112-Symp 8:45 am
CAV1.3 L-TYPE CALCIUM CHANNEL DYSFUNCTION IN HU-
MAN DISEASE. Jörg Striessnig
1113-Symp 9:15 am
REGULATION OF VOLTAGE-GATED CALCIUM CHANNEL
TRAFFICKING AND FUNCTION BY AUXILIARY SUBUNITS.
Annette C. Dolphin
1114-Symp 9:45 am
3D STRUCTURES OF THE CALCIUM RELEASE-ACTIVATED
CALCIUM CHANNEL ORAI. Stephen B. Long
8:15 am–10:15 am, Room 135
Symposium
Force Sensing in Muscle
Co-Chairs
Mathias Gautel, King’s College London, United Kingdom
Gabriella Piazzesi, University of Florence, Italy
1115-Symp 8:15 am
THE ELASTICITY OFTHE MYOSINMOTOR AND
MYOFILAMENTS IN THE MUSCLE SARCOMERE.
Gabriella Piazzesi, Luca Fusi, Marco Caremani, Elisabetta Brunello,
Massimo Reconditi, Luca Melli, Marco Linari, Malcolm Irving,
Vincenzo Lombardi
1116-Symp 8:45 am
EXPERIMENTAL AND COMPUTATIONAL APPROACHES
TO STUDY MYOFILAMENT STRUCTURE-FUNCTION IN
NORMAL AND DISEASED MUSCLE. Michael Regnier,
Yuanhua Cheng, Pete Kekenes-Huskey, Steffen Lindert,
Andrew McCulloch