69
Biophysical Society 58
th
Annual Meeting, San Francisco, California
M
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8:15
am
–10:15
am
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306
Platform
DNA Replication, Recombination, and Repair
Co-Chairs
Marcia Levitus, Arizona State University
Susan Tsutakawa, Lawrence Berkeley National Laboratory
1159-P
lat
8:15
am
IDENTIFICATION OF THE TRANSLOCATION STEP OF A
REPLICATIVE DNA POLYMERASE. Jose A. Morin, Francisco J. Cao,
Jose M. Lázaro, Margarita Salas, Jose M. Valpuesta, Jose L. Carrascosa,
Borja Ibarra
1160-P
lat
8:30
am
SINGLE MOLECULE STUDIES OF DNA REPLICATION
PROCESSIVITY CLAMPS.
Jennifer K. Binder
, Suman Ranjit,
Manas Chakraborty, David Kanno, Lauren Douma, Linda Bloom,
Marcia Levitus
1161-P
lat
8:45
am
DEFINING THE SUPERFAMILY CONSERVED MECHANISM FOR
FLAP ENDONUCLEASES FEN1 AND XPG SPECIFICITY FOR 5’
FLAP DNA AND DNA BUBBLES, RESPECTIVELY, BY HYBRIDS
METHODS OF CRYSTALLOGRAPHY, SAXS, EM, AND COMPU-
TATION.
Susan Tsutakawa
, Andrew Arvai, Altaf Sarker,
Jordi Querol-Audi, David Finger, Eva Nogales, Ivaylo Ivanov, Priscilla
Cooper, Jane Grasby, John Tainer
1162-P
lat
9:00
am
ROADBLOCKS ON THE E.COLI GENOME: THE WORKINGS
OF A MOLECULAR MOUSE TRAP AT THE SINGLE-MOLECULE
LEVEL.
Bojk A. Berghuis
, David Dulin, Bronwen Cross,
Nicholas E. Dixon, Nynke H. Dekker
1163-P
lat
9:15
am
SLIDING, PAUSING AND BRIDGING: HOWHUMAN XRCC4 AND
XLF INTERACTWITHDNA. Andrea Candelli, Gerrit Sitters, Ineke Brouwer,
Stephanie Heerema, Mauro Modesti, Erwin Peterman,
Gijs J.Wuite
1164-P
lat
9:30
am
SINGLE DNA GLYCOSYLASE MOLECULES DIFFUSE ONE-
DIMENSIONALLY ANDUSE AWEDGE RESIDUETO PROBE FOR
OXIDATIVELY DAMAGED BASES.
Shane Nelson
, Andrew Dunn,
Susan S. Wallace, David M. Warshaw
1165-P
lat
9:45
am
ONE ORC WITH MANY FACES.
Huilin Li
1166-P
lat
10:00
am
INTERACTIONS BETWEEN THE
E. COLI
SOS RESPONSE
PROTEIN UMUD AND DNA POLYMERASE III ALPHA SUBUNIT
HAVE IMPLICATIONS FOR REGULATING REPLICATION IN
RESPONSE TO DNA DAMAGE.
Penny Beuning
, Michelle C. Silva,
Philip Nevin, Kathy R. Chaurasiya, Clarissa Ruslie, Lukas Voortman,
Samer Lone, Erin A. Ronayne, Mark C. Williams
8:30
am
–10:00
am
, R
oom
122
Minority Affairs Committee Meeting
9:00
am
–10:30
am
, R
oom
123
Exhibitor Presentation
Park Systems, Inc.
New Door to Live Single Cell Research
Atomic Force Microscopy (AFM) is a powerful measurement technique for
nanoscale science. AFM is able to provide high-resolution imaging of biological
structures below the optical limit, as well as the monitoring of the dynamics
in biological systems and processes under physiological conditions; however,
certain limitations for AFM still exist in the field of bio-applications. In recent
times, the development of another kind of scanning probe microscopy (SPM)
technique, scanning ion conductance microscopy (SICM), has overcome these
limitations and enabled noninvasive, nanoscale investigation of live cells. SICM
applications include imaging of cell topography, monitoring of live cell dynam-
ics, mechanical stimulation of live cells, surface patterning, and so forth.
We at Park Systems have developed AFM for advanced nanoscale metrology,
which separates the z-scanner from the x-y scanner. An independent z-scanner
also provides an excellent platform for developing other SPM techniques such as
SICM. In addition, the platform which separates the z-scanner from x-y scanner,
enables us to easily switch between an AFM and an SICM z-scanner to apply
both techniques without moving samples. The common glass micropipette is
used in SICM as the sensitive probe, instead of a silicon-based stylus, and can
glide over live cells while maintaining an absolute non-contact imaging mode.
Its electrochemical current feedback system further enhances biological sample
imaging. Combining confocal fluorescence data to the SICM 3D data, using an
image overlay feature, provides even more data about structure of cells as related
to their membranes. These advances of convergence in instrumentation will be
utilized in various kinds of biomedical research and become a new driving force
for biophysics and nanobioscience.
Presenter:
Sangjoon Cho, Sr. Director of R&D, Park Systems, Inc.
10:00
am
–11:00
am
, R
oom
300
Career Center Workshop
Career Open Forum/Career Q&A Session
Bring your coffee and start your day with this industry-focused forum, in-
tended to answer any/all of your questions related to your job search. Space
is limited! This is your opportunity to explore the myths and realities associ-
ated with an effective job search. Come with any and all job search-related
questions and we’ll do our best to provide answers and guidance.
10:00
am
–5:00
pm
, H
all
D
Biomolecular Discovery Dome
10:00
am
–5:00
pm
, H
all
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Exhibits
10:15
am
–11:00
am
, H
all
D
Coffee Break
10:15
am
–11:15
am
, R
oom
302
New Member Welcome Coffee
All new Biophysical Society members are invited to participate in an informal
gathering to meet members of the Society’s council and committees, find out
about the Society’s activities, get acquainted with other new members, and enjoy
refreshments. Current members are encouraged to come meet the newmembers.
10:45
am
–12:45
pm
, R
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134
Symposium
Biophysics of Personalized Medicine
Co-Chairs
Donald Engelman, Yale University
Kathleen Giacomini, University of California, San Francisco
1167-S
ymp
10:45
am
IMAGING ANDTREATINGTUMORS BYTARGETINGTHEIR
ACIDITYWITH PHLIP, A PH-SENSITVE INSERTION PEPTIDE.
DonaldM. Engelman
, Ming An, Oleg A. Andreev, Francisco N. Barrera,
Raman Bahal, Marcus W. Bosenberg, Christopher Cheng, Peter M. Glazer,
Alexander Karabadzhak, Yana K. Reshetnyak, W. Mark Saltzman,
Frank J. Slack, Alexander A. Svoronos, DamienThevenin
