72
Biophysical Society 58
th
Annual Meeting, San Francisco, California
10:45
am
–12:45
pm
, R
oom
306
Platform
Structure and Dynamics of RNA in Biology
Co-Chairs
Peter Cornish, University of Missouri
Edward O’Brien, Cambridge University, United Kingdom
1210-P
lat
10:45
am
DECIPHERING RIBOSOMAL FRAMESHIFTING DYNAMICS.
Shannon Yan
, Jin-Der Wen, Laura Lancaster, Harry Noller,
Carlos Bustamante, Ignacio Tinoco, Jr.
1211-P
lat
11:00
am
UNRAVELING THE MYSTERY OF RIBOSOME INDUCED RNA
UNFOLDING.
Peter Cornish
, Peiwu Qin, Dongmei Yu
1212-P
lat
11:15
am
SINGLE-MOLECULE PROFILINGOF RIBOSOMETRANSLATIONAL
PHENOMENA.
Jin Chen
, Alexey Petrov, Magnus Johansson,
Albert Tsai, Seán E. O’Leary, Joseph D. Puglisi
1213-P
lat
11:30
am
THE RIBOSOME USES COOPERATIVE CONFORMATIONAL
CHANGES TO MAXIMIZE THE EFFICIENCY OF PROTEIN
SYNTHESIS. Wei Ning, Jingyi Fei,
Ruben L. Gonzalez, Jr.
1214-P
lat
11:45
am
ROTATIONAL MOTIONS OF DOMAINS IN ELONGATION
FACTOR G DETECTED BY SINGLE-MOLECULE POLARIZED
FLUORESCENCE MICROSCOPY.
Chunlai Chen
, Xiaonan Cui,
John F. Beausang, Barry S. Cooperman, Yale E. Goldman
1215-P
lat
12:00
pm
REFINING CRYSTAL STRUCTURES AGAINST CRYO-EM DATA
USING MOLECULAR DYNAMICS SIMULATIONS TO OBTAIN
A COMPLETE ATOMISTIC PATHWAY OF TRANSFER RNA
TRANSLOCATION. Andrea C. Vaiana, Carsten Kutzner, Lars V. Bock,
Christian Blau,
Helmut Grubmuller
1216-P
lat
12:15
pm
THE EFFECT OF CODON TRANSLATION RATES ON
COTRANSLATIONAL PROTEIN FOLDING MECHANISMS
OF ARBITRARY COMPLEXITY.
Edward P. O’Brien
1217-P
lat
12:30
pm
PROTEIN SYNTHESIS BY RIBOSOMES: MAPPING IN VITRO
ONTO IN VIVO RATES.
Sophia Rudorf
, Michael Thommen,
Marina V. Rodnina, Reinhard Lipowsky
11:00
am
–12:30
pm
, R
oom
123
Exhibitor Presentation
Nanion Technologies
Workshop on Automated Patch Clamp: From Single Channels,
Primary Cells, Action Potentials to 384 Giga-Seal Recordings in a
Parallel HTS Format
The Port-a-Patch recently turned 10 years old, and is going stronger than ever.
It’s still the smallest patch clamp rig in the world, and makes patch clamp re-
cordings accessible to anyone spending a couple of hours with it. Giga-seal re-
cordings and the excellent voltage-clamp of the cellular membrane ensure high
quality data, and the Port-a-Patch add-ons allow unprecedented experimental
freedom, including temperature control, internal perfusion, automated action
potential recordings, and recordings from primary and stem cell-derived cells.
Recently, the Port-a-Patch technology was scaled up to eight simultaneous
recordings (Patchliner), maintaining the same data quality and experimental
possibilities, and now we did it again: 384 Port-a-Patches have been shrunk to
fit inside a shoebox – called the Patch Engine (PE). Two Patch Engines can be
integrated per SyncroPatch 384PE platform, allowing for patch clamp-based
ion channel HTS from up to 768 cells in parallel, and we will tell you more
about it during this workshop.
Another topic for the workshop is the bilayer-reconstitution of ion chan-
nels and nanopores, efficiently investigated using the Orbit 16, a parallel
device for formation of and recordings for up to 16 artificial bilayers at
once. Using Micro Electrode Cavity Array (MECA, Ionera), a 4 x 4 array
of circular micro-cavities in a highly inert polymer, the bilayer is automati-
cally formed by remotely actuated painting (Ionera- SPREAD).
Welcome to our workshop and learn from live, hands-on experiments on
the Port-a-Patch and Orbit 16, and let us show you how to scale up your
ion channel screening project to HTS-standards!
Presenters:
Niels Fertig, CEO, Nanion Technologies
Andrea Brüggemann, CSO, Nanion Technologies
Gerhard Baaken, Ionera
11:30
am
–12:30
pm
, R
oom
300
Career Center Workshop
Beyond the Bench: Preparing for Your Career
Transition in the Life Sciences
There are numerous alternative career options for the seasoned bench
scientist who may have decided to take his/her talents and apply them in
a new direction. This transition can be accomplished without having to
matriculate in another graduate program, and this session explores the
how’s and why’s of making such a transition. Be prepared to talk about the
role you are thinking about moving into, why you may have chosen this
alternative path, and what successes you may have had thus far.
11:30
am
–1:00
pm
, R
oom
308
Undergraduate Student Pizza “Breakfast”
The Education Committee is hosting this “breakfast” for undergraduate
students. This session provides a valuable networking and social opportunity
for undergraduate student attendees to meet other students and Committee
members, to discuss academic goals and questions, and to develop a biophysics
career path. The Emily Gray Awardee will also give a talk at this event. Limited
to the first 100 attendees.
Speaker:
Alberto Diaspro, Italian Institute of Technology
1:00
pm
–2:30
pm
, R
oom
123
Exhibitor Presentation
World Precision Instruments, Inc.
Applications in Biophysics Utilizing World Precision Instrument’s
(WPI) New Biofluorometer
Introduction to WPI’s New Biofluorometer with high-power LED
modules. Potential applications and experimental design will be discussed
in the field of Biophysics, including integration with Muscle Physiology
experiments and microscopy systems for general fluorescence applications.
Presenter:
Mathias Belz, Director of Optics, World Precision Instruments, Inc.
1:00
pm
–3:00
pm
, H
all
D
Graduate and Postdoc Institution Fair
This fair introduces students and postdoctoral candidates to colleges and
universities with leading programs in biophysics. Open to all attendees.
