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QUALITY CONTROL DEPARTMENT
Name of Sample
: Withanolides : std mix
Method Name
: C:\Methods\WITHANIA.met
Data File Name
: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\SM-01-Rep1
Vial No
: 10001
Injection Volume
: 20
Date & Time
: 4/3/07 7:44:32 PM
Minutes
0
5
10
15
20
25
30
35
40
45
Volts
0.0
0.2
0.4
0.6
0.8
17.233
21.400
22.058
23.033
23.892
27.008
Detector A - 1 (227nm)
Pk # Retention Time Name
Area Area Percent
1
17.233
Withanoside IV
575205
7.35
2
21.400
Withanoside V
274773
3.51
3
22.058
Withaferin A
1813207
23.18
4
23.033
Withastramonolide
1788787
22.86
5
23.892
Withanolide A
1849418
23.64
6
27.008
Withanolide B
1522047
19.45
Totals
7823437
100.00